GLYR1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

GLYR1 Polyklonal Antikörper für IF, IHC, IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, CoIP, ChIP, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 14833-1-AP

Synonyme

BM045, Glyoxylate reductase 1 homolog, GLYR1, HIBDL, N PAC, N-PAC, Nuclear protein NP60, Nuclear protein of 60 kDa, Putative oxidoreductase GLYR1

---

Galerie der Validierungsdaten

• 

  WB analysis using 14833-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14833-1-AP (GLYR1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HL-60 using 14833-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 14833-1-AP (GLYR1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 14833-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14833-1-AP (GLYR1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 14833-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 14833-1-AP (GLYR1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of K-562 using 14833-1-AP

  IP Result of anti-GLYR1 (IP:14833-1-AP, 4ug; Detection:14833-1-AP 1:500) with K-562 cells lysate 3200ug.

• 

  IHC staining of mouse testis using 14833-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 14833-1-AP (GLYR1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 14833-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 14833-1-AP (GLYR1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 14833-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14833-1-AP (GLYR1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 14833-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14833-1-AP (GLYR1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 14833-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 14833-1-AP (GLYR1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human colon using 14833-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 14833-1-AP (GLYR1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 14833-1-AP

  Immunofluorescent analysis of Hela cells, using N-PAC antibody 14833-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HL-60-Zellen, HeLa-Zellen, Jurkat-Zellen
• Erfolgreiche IP: K-562-Zellen
• Erfolgreiche Detektion in IHC: Maushodengewebe, humanes Kolongewebe, humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:5000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• CoIP: See 1 publications below
• ChIP: See 1 publications below

Produktinformation

14833-1-AP bindet in WB, IP, IHC, IF, CoIP, ChIP, ELISA GLYR1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GLYR1 fusion protein Ag6637
• Vollständiger Name: cytokine-like nuclear factor n-pac
• Berechnetes Molekulargewicht: 61 kDa
• Beobachtetes Molekulargewicht: 60 kDa
• GenBank-Zugangsnummer: BC064940
• Gene symbol: N-PAC
• Gene ID (NCBI): 84656
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human		Cell; Transcription factor protein interactomes reveal genetic determinants in heart disease.; Authors - Barbara Gonzalez-Teran
mouse	ChIP, WB	Genomics Proteomics Bioinformatics; Npac Is a Co-factor of Histone H3K36me3 and Regulates Transcriptional Elongation in Mouse Embryonic Stem Cells.; Authors - Sue Yu
human	WB,IHC,CoIP,IF	J Exp Clin Cancer Res; Downregulation of GLYR1 contributes to microsatellite instability colorectal cancer by targeting p21 via the p38MAPK and PI3K/AKT pathways.; Authors - Zhiyan Hu ; KD Validated