MYH2-specific Monoklonaler Antikörper

MYH2-specific Monoklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

2

CloneNo.

1F1B6

Kat-Nr. : 66212-1-Ig

Synonyme

MYH2, MYH2A, MYH2-specific, MYHas8, MyHC 2A, MyHC IIa, MYHSA2, Myosin 2, Myosin 2a, Myosin heavy chain 2, Myosin heavy chain 2a, Myosin heavy chain IIa

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Galerie der Validierungsdaten

• 

  WB analysis of human skeletal muscle using 66212-1-Ig

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 66212-1-Ig (Myosin 2a antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse heart using 66212-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 66212-1-Ig (MYH2-specific antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 66212-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 66212-1-Ig (MYH2-specific antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat skeletal muscle using 66212-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue slide using 66212-1-Ig (MYH2-specific antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skeletal muscle using 66212-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 66212-1-Ig (MYH2-specific antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skeletal muscle using 66212-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 66212-1-Ig (MYH2-specific antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse tongue using 66212-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse tongue tissue slide using 66212-1-Ig (MYH2-specific antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skeletal muscle using 66212-1-Ig

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 66212-1-Ig (Myosin 2a Antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of mouse tongue using 66212-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse tongue tissue slide using 66212-1-Ig (MYH2-specific antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skeletal muscle using 66212-1-Ig

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 66212-1-Ig (Myosin 2a Antibody) at dilution of 1:100 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Skelettmuskelgewebe
• Erfolgreiche Detektion in IHC: Mausherzgewebe, humanes Skelettmuskelgewebe, Maus-Skelettmuskelgewebe, Mauszungengewebe, Ratten-Skelettmuskelgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:100000
• Immunhistochemie (IHC): IHC : 1:2000-1:8000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below

Produktinformation

66212-1-Ig bindet in WB, IHC, ELISA MYH2-specific und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: myosin, heavy chain 2, skeletal muscle, adult
• Berechnetes Molekulargewicht: 223 kDa
• Beobachtetes Molekulargewicht: 200 kDa
• GenBank-Zugangsnummer: NM_017534
• Gene symbol: MYH2
• Gene ID (NCBI): 4620
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MYH2 (Myosin II) is a member of the class II or conventional myosin heavy chains. Myosin II was first isolated from muscle but is also found in non muscle cells, and it is especially enriched in highly motile cell types such as amoebae. It forms bipolar filaments that interact with actin filaments to produce contraction. And it is the motor protein that generates force to drive muscle contraction. Functions in skeletal muscle contraction. The antibody is specific to MYH2, will not bind other myosins. It is a skeletal muscle specific antibody.

Publikationen

Species	Application	Title
	WB	J Agric Food Chem; Spermidine-Activated Satellite Cells Are Associated with Hypoacetylation in ACVR2B and Smad3 Binding to Myogenic Genes in Mice.; Authors - Luchu Zhang
	WB	Sci Rep; Trimetazidine and exercise provide comparable improvements to high fat diet-induced muscle dysfunction through enhancement of mitochondrial quality control.; Authors - Wenliang Zhang