Myogenin Monoklonaler Antikörper

Myogenin Monoklonal Antikörper für WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Ratte und mehr (4)

Anwendung

WB, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

13

CloneNo.

1C10B9

Kat-Nr. : 67082-1-Ig

Synonyme

bHLHc3, Myf 4, MYF4, MYOG, Myogenic factor 4, MYOGENIN, myogenin (myogenic factor 4)

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Galerie der Validierungsdaten

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  WB analysis of human skeletal muscle using 67082-1-Ig

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 67082-1-Ig (Myogenin antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 67082-1-Ig

  human heart tissue were subjected to SDS PAGE followed by western blot with 67082-1-Ig (Myogenin antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig heart using 67082-1-Ig

  pig heart tissue were subjected to SDS PAGE followed by western blot with 67082-1-Ig (Myogenin antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat heart using 67082-1-Ig

  rat heart tissue were subjected to SDS PAGE followed by western blot with 67082-1-Ig (Myogenin antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Skelettmuskelgewebe, Hausschwein-Herzgewebe, humanes Herzgewebe, Rattenherzgewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 13 publications below
• IF: See 3 publications below

Produktinformation

67082-1-Ig bindet in WB, IF, ELISA Myogenin und zeigt Reaktivität mit Hausschwein, human, Ratten

• Getestete Reaktivität: Hausschwein, human, Ratte
• In Publikationen genannte Reaktivität: human, Kaninchen, Maus, Rind, Ziege
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Myogenin fusion protein Ag6414
• Vollständiger Name: myogenin (myogenic factor 4)
• Berechnetes Molekulargewicht: 25 kDa
• Beobachtetes Molekulargewicht: 26 kDa
• GenBank-Zugangsnummer: BC053899
• Gene symbol: MYOG
• Gene ID (NCBI): 4656
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20℃ lagern. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MYOG (encoded Myogenin protein) is a muscle-specific transcription factor which belongs to a family of basic helix-loop-helix transcription factors. It plays a role in muscle differentiation, cell cycle exit and muscle atrophy(PMID: 30315160). It can be initiate the myogenic program in myoblasts, fiblasts and veritey of other cell types. Diseases associated with MYOG include Embryonal Rhabdomyosarcoma and Gallbladder Sarcoma.

Publikationen

Species	Application	Title
mouse	WB	J Cachexia Sarcopenia Muscle; circRNAome profiling reveals circFgfr2 regulates myogenesis and muscle regeneration via a feedback loop.; Authors - Junyu Yan
human	WB	Mol Ther Nucleic Acids; Circular RNA RBPMS inhibits bladder cancer progression via miR-330-3p/RAI2 regulation.; Authors - Chen Yang
mouse	WB	Int J Mol Sci; G6PD Deficiency Is Crucial for Insulin Signaling Activation in Skeletal Muscle.; Authors - Aiwen Jiang
mouse	WB	Front Cell Dev Biol; Phosphorylation of Dynamin-Related Protein 1 (DRP1) Regulates Mitochondrial Dynamics and Skeletal Muscle Wasting in Cancer Cachexia.; Authors - Xiangyu Mao
goat	WB	FASEB J; Circular RNA circUSP13 sponges miR-29c to promote differentiation and inhibit apoptosis of goat myoblasts by targeting IGF1.; Authors - Zhen Zhang
	WB	FASEB J; MicroRNA profiling reveals miR-145-5p inhibits goat myoblast differentiation by targeting the coding domain sequence of USP13.; Authors - Zhen Zhang