Mouse IgG2b isotype control Monoklonaler Antikörper

Mouse IgG2b isotype control Monoklonal Antikörper für FC, IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

Megathura crenulata

Anwendung

WB, IHC, FC, chIP, ELISA

Konjugation

Unkonjugiert

Publikationen

4

CloneNo.

11B8C4

Kat-Nr. : 66360-3-Ig

Synonyme

Mouse IgG2b, Mouse IgG2b isotype control

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• Galerie der Validierungsdaten
• Proteintech vs. Other Vendors

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  WB analysis using 66360-3-Ig

  Various lysates (50 ug/lane) were subjected to SDS PAGE followed by western blot with 66360-3-Ig (Mouse IgG2b isotype control antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon using 66360-3-Ig

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66360-3-Ig (Mouse IgG2b isotype control antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 66360-3-Ig

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66360-3-Ig (Mouse IgG2b isotype control antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  FC experiment of Jurkat using 66360-3-Ig

  1X10^6 Jurkat cells were stained with 0.20ug Mouse IgG2b isotype control antibody (66360-3-Ig, red) or control antibody (blue). Fixed with 90% MeOH.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IHC: Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:1500-1:6000
• Durchflusszytometrie (FC): FC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• ChIP: See 2 publications below

Produktinformation

66360-3-Ig bindet in WB, IHC, FC, chIP, ELISA Mouse IgG2b isotype control und zeigt Reaktivität mit Megathura crenulata

• Getestete Reaktivität: Megathura crenulata
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Rekombinantes Protein
• Vollständiger Name: Mouse IgG2b isotype control
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

This antibody is used as the isotype control of mouse IgG2b. It can be used as the isotype control in Flow Cytometry, Immuno-Precipitation and other experiments. The immunogen of this antibody is Keyhole Limpet Hemocyanin (KLH).

Publikationen

Species	Application	Title
	ChIP	Front Oncol; The COX10-AS1/miR-641/E2F6 Feedback Loop Is Involved in the Progression of Glioma.; Authors - Liang Liu
	chIP	Cell Death Dis; E2F1 mediated DDX11 transcriptional activation promotes hepatocellular carcinoma progression through PI3K/AKT/mTOR pathway.; Authors - Yan Yu
	WB	Aging Dis; Age-related Loss of miR-124 Causes Cognitive Deficits via Derepressing RyR3 Expression; Authors - Kai Liu
		Immunity; Ribosome-rescuer PELO catalyzes the oligomeric assembly of NOD-like receptor family proteins via activating their ATPase enzymatic activity; Authors - Xiurong Wu