Mmp9 Polyklonaler Antikörper

Mmp9 Polyklonal Antikörper für IF, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IF, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 30592-1-AP

Synonyme

AW743869, B/MMP9, Clg4b, matrix metallopeptidase 9, MMP 9, Mmp9, pro MMP 9

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Galerie der Validierungsdaten

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  WB analysis using 30592-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30592-1-AP (Mmp9 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  IF Staining of A431 using 30592-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using Mmp9 antibody (30592-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of U2OS using 30592-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using Mmp9 antibody (30592-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mauslungengewebe
• Erfolgreiche Detektion in IF: U2OS-Zellen, A431-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:16000
• Immunfluoreszenz (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

30592-1-AP bindet in WB, IF, ELISA Mmp9 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Rekombinantes Protein
• Vollständiger Name: matrix metallopeptidase 9
• Berechnetes Molekulargewicht: 81kd
• Beobachtetes Molekulargewicht: 80-100 kDa
• GenBank-Zugangsnummer: NM_013599
• Gene symbol: Mmp9
• Gene ID (NCBI): 17395
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Mmp9 (matrix metallopeptidase 9), also known as Clg4b. It is expected to be located inextracellular matrix. Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, tissue remodeling, and disease processes, such as arthritis or metastasis. Studies in rhesus monkeys suggest that MMP9 is involved in IL-8-induced mobilization hematopoietic progenitor cells from bone marrow, and murine studies suggest a role in tumor-associated tissue remodeling. The calculated molecular weight of Mmp9 is 80 kDa, the pro-MMP9 is 92 kDa, and this protein can exist as a dimer of 180 kDa (PMID:7492685).