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  • Featured Product
  • KD/KO Validated

Mitofilin Polyklonaler Antikörper

Mitofilin Polyklonal Antikörper für FC (Intra), IF

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

IF, FC (Intra)

Konjugation

CoraLite®594 Fluorescent Dye

Kat-Nr. : CL594-10179

Synonyme

DKFZp779P1653, HMP, IMMT, Mitochondrion Marker, Mitofilin, P87, P87/89, P89, PIG4, PIG52

Galerie der Validierungsdaten

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite®594-conjugated Mitofilin Polyclonal antib (CL594-10179)

    IF Staining of HeLa using CL594-10179

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite®594 Mitofilin antibody (CL594-10179) at dilution of 1:100.

  • Flow cytometry (FC) experiment of HEK-293T cells using CoraLite®594-conjugated Mitofilin Polyclonal antib (CL594-10179)

    FC experiment of HEK-293T using CL594-10179

    1X10^6 HEK-293T cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human Mitofilin (CL594-10179) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Geprüfte Anwendungen

Erfolgreiche Detektion in IFHeLa-Zellen
Erfolgreiche Detektion in FCHEK-293T-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Immunfluoreszenz (IF)IF : 1:50-1:500
Durchflusszytometrie (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

CL594-10179 bindet in IF, FC (Intra) Mitofilin und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen Mitofilin fusion protein Ag0102
Vollständiger Name inner membrane protein, mitochondrial (mitofilin)
Berechnetes Molekulargewicht 90 kDa
Beobachtetes Molekulargewicht 80-90 kDa
GenBank-ZugangsnummerBC002412
Gene symbol IMMT
Gene ID (NCBI) 10989
Konjugation CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
LagerungsbedingungenBei -20°C lagern. Vor Licht schützen. Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

IMMT (also known as mitofilin) is an inner mitochondrial membrane protein that is preferentially expressed in heart tissue and is commonly used as the marker for mitochondria. Mitofilin is known to be a critical organizer of mitochondrial cristae morphology and is indispensable for normal mitochondrial function. Three isoforms of mitofilin exist due to the alternative splicing. All three proteins of 87kD, 89kD and 80kD can be detected in immunoblot analysis with this antibody.

Protokolle

Produktspezifische Protokolle
IF protocol for CL594 Mitofilin antibody CL594-10179Protokoll herunterladen
FC protocol for CL594 Mitofilin antibody CL594-10179Protokoll herunterladen
Standard-Protokolle
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