Mecr Polyklonaler Antikörper

Mecr Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

5

Kat-Nr. : 51027-2-AP

Synonyme

AI195831, Mecr, Nrbf1

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Galerie der Validierungsdaten

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  WB analysis using 51027-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 51027-2-AP (Mecr antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 51027-2-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 51027-2-AP (Mecr antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse heart using 51027-2-AP

  IP Result of anti-Mecr (IP:51027-2-AP, 4ug; Detection:51027-2-AP 1:1500) with mouse heart tissue lysate 4800ug.

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  IHC staining of human ovary tumor using 51027-2-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 51027-2-AP (Mecr antibody) at dilution of 1:100 (under 10x lens).

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  IF Staining of C2C12 using 51027-2-AP

  Immunofluorescent analysis of (4% PFA) fixed C2C12 cells using Mecr antibody (51027-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausherzgewebe, Maus-Magengewebe, Maus-Skelettmuskelgewebe, Rattenherzgewebe
• Erfolgreiche IP: Mausherzgewebe
• Erfolgreiche Detektion in IF: C2C12-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

51027-2-AP bindet in WB, IP, IHC, IF, ELISA Mecr und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hefe, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Mecr fusion protein Ag0465
• Vollständiger Name: mitochondrial trans-2-enoyl-CoA reductase
• Berechnetes Molekulargewicht: 40 kDa
• Beobachtetes Molekulargewicht: 37-40 kDa
• GenBank-Zugangsnummer: BC003864
• Gene symbol: Mecr
• Gene ID (NCBI): 26922
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Mitochondrial Trans-2-Enoyl-CoA Reductase is abbrivated as MECR, involved in human mitochonrial fatty acid synthesis(mtFAS) as an oxidoreductase. (PMID: 27817865) MECR has molecular weight can be calculated around 37KDa. With immunopercipitation reaction on mouse heart samples, the IgG heavy chain, dimer of MECR is around 65KDa. Numerous studies have shown disorder of lipid metabolism is closely related with malignance, especially in liver cancer. As futher research suggests that MECR has close realitionship with cell prolifereation, appotosis, and metastasis (PMID: 31178528)

Publikationen

Species	Application	Title
human	WB	Am J Hum Genet; MECR Mutations Cause Childhood-Onset Dystonia and Optic Atrophy, a Mitochondrial Fatty Acid Synthesis Disorder.; Authors - Gali Heimer
mouse	WB	Elife; Mitochondrial fatty acid synthesis coordinates oxidative metabolism in mammalian mitochondria.; Authors - Sara M Nowinski
mouse	IHC,IF	J Neurosci; Impaired mitochondrial fatty acid synthesis leads to neurodegeneration in mice.; Authors - Remya R Nair
mouse	WB	Hum Mol Genet; Genetic modifications of Mecr reveal a role for mitochondrial 2-enoyl-CoA/ACP reductase in placental development in mice.; Authors - Remya R Nair
yeast	WB	Nat Commun; An engineered variant of MECR reductase reveals indispensability of long-chain acyl-ACPs for mitochondrial respiration; Authors - M Tanvir Rahman