Mcp1 Monoklonaler Antikörper

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• KD/KO Validated

Mcp1 Monoklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Maus und mehr (1)

Anwendung

WB, IHC, IF, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

120

CloneNo.

1B9F7

Kat-Nr. : 66272-1-Ig

Synonyme

AI323594, Ccl2, chemokine (C C motif) ligand 2, HC11, JE, MCAF, MCP 1, Mcp1, Scya2, Sigje, SMC CF

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Galerie der Validierungsdaten

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  WB analysis using 66272-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66272-1-Ig (Mcp1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 66272-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66272-1-Ig (Mcp1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 66272-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66272-1-Ig (Mcp1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of RAW 264.7 using 66272-1-Ig

  LPS treated RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66272-1-Ig (Mcp1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse kidney using 66272-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 66272-1-Ig (Mcp1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 66272-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 66272-1-Ig (Mcp1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse liver using 66272-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 66272-1-Ig (Mcp1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 66272-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 66272-1-Ig (Mcp1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: DC2.4-Zellen, Maushirngewebe, Mauslungengewebe, Mausnierengewebe, Maus-Plazenta-Gewebe, mit LPS behandelte RAW 264.7-Zellen
• Erfolgreiche Detektion in IHC: Mausnierengewebe, Mauslebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 90 publications below
• IHC: See 29 publications below
• IF: See 14 publications below
• CoIP: See 1 publications below

Produktinformation

66272-1-Ig bindet in WB, IHC, IF, CoIP, ELISA Mcp1 und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• In Publikationen genannte Reaktivität: Kaninchen, Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Mcp1 fusion protein Ag24085
• Vollständiger Name: chemokine (C-C motif) ligand 2
• Beobachtetes Molekulargewicht: 26 kDa
• GenBank-Zugangsnummer: NM-011333
• Gene symbol: Ccl2
• Gene ID (NCBI): 20296
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Monocyte chemoattractant protein-1 (MCP-1/CCL2) is one of the key chemokines that regulate migration and infiltration of monocytes/macrophages. Both CCL2 and its receptor CCR2 have been demonstrated to be induced and involved in various diseases. Migration of monocytes from the blood stream across the vascular endothelium is required for routine immunological surveillance of tissues, as well as in response to inflammation.

Publikationen

Species	Application	Title
	WB	Nature; A druggable copper-signalling pathway that drives inflammation; Authors - Stéphanie Solier
mouse	IF	Gut; Cross-talk between the gut microbiota and monocyte-like macrophages mediates an inflammatory response to promote colitis-associated tumourigenesis.; Authors - Yunben Yang
mouse	IF	Hepatology; Single-cell transcriptomic analysis reveals a hepatic stellate cell-activation roadmap and myofibroblast origin during liver fibrosis.; Authors - Wu Yang
mouse	IHC	Cell Death Differ; Ferroptotic stress facilitates smooth muscle cell dedifferentiation in arterial remodelling by disrupting mitochondrial homeostasis; Authors - Qing-Xin Ji
mouse	WB	Nat Commun; Deficiency of WTAP in hepatocytes induces lipoatrophy and non-alcoholic steatohepatitis (NASH); Authors - Xinzhi Li
mouse	WB	Nat Commun; Nuclear pore protein NUP210 depletion suppresses metastasis through heterochromatin-mediated disruption of tumor cell mechanical response.; Authors - Ruhul Amin