Mcp1 Polyklonaler Antikörper

Mcp1 Polyklonal Antikörper für IF, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

6

Kat-Nr. : 26161-1-AP

Synonyme

AI323594, Ccl2, chemokine (C C motif) ligand 2, HC11, JE, MCAF, MCP 1, Mcp1, Scya2, Sigje, SMC CF

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Galerie der Validierungsdaten

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  WB analysis using 26161-1-AP

  Untreated RAW 264.7 cells, RAW 264.7 cells (treated with 1 μg/ml LPS 20 hours then add 300 ng/ml Brefeldin A for 4hours) were subjected to SDS PAGE followed by western blot with 26161-1-AP (Mcp1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 26161-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 26161-1-AP (Mcp1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: mit LPS und Brefeldin A behandelte RAW 264.7-Zellen
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IHC: See 2 publications below
• IF: See 3 publications below

Produktinformation

26161-1-AP bindet in WB, IHC, IF, ELISA Mcp1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Mcp1 fusion protein Ag24085
• Vollständiger Name: chemokine (C-C motif) ligand 2
• Beobachtetes Molekulargewicht: 16-20 kDa
• GenBank-Zugangsnummer: NM-011333
• Gene symbol: Ccl2
• Gene ID (NCBI): 20296
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Monocyte chemoattractant protein-1 (MCP-1/CCL2) is one of the key chemokines that regulate migration and infiltration of monocytes/macrophages. Both CCL2 and its receptor CCR2 have been demonstrated to be induced and involved in various diseases. Migration of monocytes from the blood stream across the vascular endothelium is required for routine immunological surveillance of tissues, as well as in response to inflammation.

Publikationen

Species	Application	Title
rat	WB	Int J Mol Sci; Enhanced Activation of the S1PR2-IL-1β-Src-BDNF-TrkB Pathway Mediates Neuroinflammation in the Hippocampus and Cognitive Impairment in Hyperammonemic Rats; Authors - María Sancho-Alonso
mouse	IF	Front Cell Neurosci; Effects of Ischemia on the Migratory Capacity of Microglia Along Collagen Microcontact Prints on Organotypic Mouse Cortex Brain Slices.; Authors - Katharina Steiner
mouse	IHC	Pharmaceutics; Targeted Therapy of Acute Liver Injury via Cryptotanshinone-Loaded Biomimetic Nanoparticles Derived from Mesenchymal Stromal Cells Driven by Homing; Authors - Xin Zhang
mouse	IF	J Biol Chem; rhFGF-21 accelerates corneal epithelial wound healing through the attenuation of oxidative stress and inflammatory mediators in diabetic mice; Authors - Le Li
mouse	IF	Proc Natl Acad Sci U S A; Bacterial indole-3-lactic acid affects epithelium-macrophage crosstalk to regulate intestinal homeostasis; Authors - Kaiyuan Yu
mouse	IHC	Phytomedicine; Cuyun Recipe ameliorates pregnancy loss by regulating macrophage polarization and hypercoagulable state during the peri-implantation period in an ovarian hyperstimulation mouse model; Authors - Wenxin Zhu