MYPT1 Monoklonaler Antikörper

MYPT1 Monoklonal Antikörper für IF, WB, ELISA

Wirt / Isotyp

Maus / IgG3

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

1

CloneNo.

2A1A9

Kat-Nr. : 66506-1-Ig

Synonyme

MBS, MYPT1, PPP1R12A

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Galerie der Validierungsdaten

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  WB analysis of Raji using 66506-1-Ig

  Raji cells were subjected to SDS PAGE followed by western blot with 66506-1-Ig (MYPT1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 66506-1-Ig

  K-562 cells were subjected to SDS PAGE followed by western blot with 66506-1-Ig (MYPT1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 66506-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 66506-1-Ig (MYPT1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 66506-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66506-1-Ig (MYPT1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 66506-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66506-1-Ig (MYPT1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HeLa using 66506-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using MYPT1 antibody (66506-1-Ig, Clone: 2A1A9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Raji-Zellen, HEK-293-Zellen, HeLa-Zellen, Jurkat-Zellen, K-562-Zellen
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:12000
• Immunfluoreszenz (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

66506-1-Ig bindet in WB, IF, ELISA MYPT1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: Ratte
• Wirt / Isotyp: Maus / IgG3
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: MYPT1 fusion protein Ag17496
• Vollständiger Name: protein phosphatase 1, regulatory (inhibitor) subunit 12A
• Berechnetes Molekulargewicht: 1030 aa, 115 kDa
• Beobachtetes Molekulargewicht: 115 kDa
• GenBank-Zugangsnummer: BC111752
• Gene symbol: PPP1R12A
• Gene ID (NCBI): 4659
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Myosin phosphatase target subunit 1(MYPT1), which is also called PPP1R12A, is one of the subunits of myosin phosphatase. Myosin phosphatase regulates the interaction of actin and myosin downstream of the guanosine triphosphatase Rho. The small guanosine triphosphatase Rho is implicated in myosin light chain (MLC) phosphorylation, which results in contraction of smooth muscle and interaction of actin and myosin in nonmuscle cells. The guanosine triphosphate (GTP)-bound, active form of RhoA (GTP.RhoA) specifically interacted with the myosin-binding subunit (MBS) of myosin phosphatase, which regulates the extent of phosphorylation of MLC. Rho-associated kinase (Rho-kinase), which is activated by GTP. RhoA, phosphorylated MBS and consequently inactivated myosin phosphatase. Overexpression of RhoA or activated RhoA in NIH 3T3 cells increased phosphorylation of MBS and MLC. Thus, Rho appears to inhibit myosin phosphatase through the action of Rho-kinase. Phosphorylation of MYPT1 at Thr696 and Thr853 results in phosphatase inhibition and cytoskeletal reorganization.

Publikationen

Species	Application	Title
rat	WB	Brain Res; The ROCK inhibitor Y-27632 ameliorates blood-spinal cord barrier disruption by reducing tight junction protein degradation via the MYPT1-MLC2 pathway after spinal cord injury in rats.; Authors - Sheng Chang