MYOM3 Polyklonaler Antikörper

MYOM3 Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

11

Kat-Nr. : 17692-1-AP

Synonyme

MYOM3, Myomesin 3, Myomesin family member 3, myomesin family, member 3, RP11 293P20.1

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Galerie der Validierungsdaten

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  WB analysis using 17692-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17692-1-AP (MYOM3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse skeletal muscle using 17692-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 17692-1-AP (MYOM3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse skeletal muscle using 17692-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 17692-1-AP (MYOM3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse skeletal muscle using 17692-1-AP

  IP result of anti-MYOM3(IP:17692-1-AP, 4ug; Detection:17692-1-AP 1:1000) with mouse skeletal muscle tissue lysate 1280 ug.

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  IHC staining of human skeletal muscle using 17692-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 17692-1-AP (MYOM3 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human skeletal muscle using 17692-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 17692-1-AP (MYOM3 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of mouse skeletal muscle using 17692-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 17692-1-AP (MYOM3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse skeletal muscle using 17692-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 17692-1-AP (MYOM3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maus-Skelettmuskelgewebe, Ratten-Skelettmuskelgewebe
• Erfolgreiche IP: Maus-Skelettmuskelgewebe
• Erfolgreiche Detektion in IHC: humanes Skelettmuskelgewebe, Maus-Skelettmuskelgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 8 publications below
• IF: See 1 publications below
• IP: See 1 publications below
• ELISA: See 1 publications below

Produktinformation

17692-1-AP bindet in WB, IP, IHC, IF, ELISA MYOM3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hund, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MYOM3 fusion protein Ag11849
• Vollständiger Name: myomesin family, member 3
• Berechnetes Molekulargewicht: 162 kDa, 136 kDa
• Beobachtetes Molekulargewicht: 162 kDa
• GenBank-Zugangsnummer: BC067101
• Gene symbol: MYOM3
• Gene ID (NCBI): 127294
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MYOM3 (myomesin 3) is a structural component of the M-band in striated muscle and is involved in sarcomere stability and resistance during intense or sustained stretching. MYOM3 can be detected mainly in intermediate speed fibers of skeletal muscle. Recently high level of MYOM3 fragments were detected in sera from patients with muscular dystrophy, including Duchenne muscular dystrophy (DMD). MYOM3 fragments may be used as serum biomarker for DMD and other neuromuscular disorders. This antibody recognizes the intact MYOM3 protein (160-160 kDa) as well as MYOM3 fragments (100 kDa and 130 kDa). (26060189)

Publikationen

Species	Application	Title
mouse	WB	J Clin Invest; A functional mini-GDE transgene corrects impairment in models of glycogen storage disease type III; Authors - Antoine Gardin
mouse	WB	Nucleic Acids Res; Palmitic acid conjugation enhances potency of tricyclo-DNA splice switching oligonucleotides.; Authors - Karima Relizani
human	WB, IP	Hum Mol Genet; Serum proteomic profiling reveals fragments of MYOM3 as potential biomarkers for monitoring the outcome of therapeutic interventions in muscular dystrophies.; Authors - Jérémy Rouillon
mouse	WB	Int J Mol Sci; Co-Administration of Simvastatin Does Not Potentiate the Benefit of Gene Therapy in the mdx Mouse Model for Duchenne Muscular Dystrophy.; Authors - Nathalie Bourg
human	IF	Stem Cell Reports; A Human Stem Cell Model of Fabry Disease Implicates LIMP-2 Accumulation in Cardiomyocyte Pathology.; Authors - Matthew J Birket
mouse	WB	PLoS One; Mutant myocilin impacts sarcomere ultrastructure in mouse gastrocnemius muscle.; Authors - Jeffrey M Lynch