MYOD1 Monoklonaler Antikörper

MYOD1 Monoklonal Antikörper für WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Publikationen

4

CloneNo.

5D1D12

Kat-Nr. : 66214-1-Ig

Synonyme

bHLHc1, Myf 3, MYF3, MYOD, MYOD1, myogenic differentiation 1, Myogenic factor 3, PUM

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Galerie der Validierungsdaten

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  WB analysis of human skeletal muscle using 66214-1-Ig

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 66214-1-Ig (MYOD1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Skelettmuskelgewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below

Produktinformation

66214-1-Ig bindet in WB, ELISA MYOD1 und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: MYOD1 fusion protein Ag13512
• Vollständiger Name: myogenic differentiation 1
• Berechnetes Molekulargewicht: 320 aa, 35 kDa
• Beobachtetes Molekulargewicht: 47 kDa
• GenBank-Zugangsnummer: BC064493
• Gene symbol: MYOD1
• Gene ID (NCBI): 4654
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MYOD1, also named as BHLHC1 or MYF3, is a 320 amino acid protein, which promotes the transcriptional activity of MYOD1 through its CDK9-mediated phosphorylation. This phosphorylation promotes its function in muscle differentiation. MYOD1 acts as a transcriptional activator that promotes transcription of muscle-specific target genes and plays a role in muscle differentiation. MYOD1 together with MYF5 and MYOG, co-occupies muscle-specific gene promoter core region during myogenesis. Induces fibroblasts to differentiate into myoblasts. he calculated molecular weight of MYOD1 is 34 kDa, but modified MYOD1 is about 45-47 kDa. (PMID: 12037670)

Publikationen

Species	Application	Title
mouse	WB	Aging (Albany NY); Vitellogenin 2 promotes muscle development and stimulates the browning of white fat; Authors - Yilei Li
human	WB	Front Oncol; Deoxypodophyllotoxin Inhibits Non-Small Cell Lung Cancer Cell Growth by Reducing HIF-1α-Mediated Glycolysis.; Authors - Yuping Yang
mouse	WB	Toxicol Appl Pharmacol; A novel oleanolic acid derivative HA-19 ameliorates muscle atrophy via promoting protein synthesis and preventing protein degradation.; Authors - Wei Cui
rat	WB	Int J Stem Cells; Synergistic Effect of Hydrogen and 5-Aza on Myogenic Differentiation through the p38 MAPK Signaling Pathway in Adipose-Derived Mesenchymal Stem Cells; Authors - Jingcheng Wang