MYO6 Polyklonaler Antikörper

MYO6 Polyklonal Antikörper für IF, IHC, IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Rezensionen (2)

Kat-Nr. : 26778-1-AP

Synonyme

DFNA22, DFNB37, KIAA0389, MYO6, myosin VI, Unconventional myosin 6

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Galerie der Validierungsdaten

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  WB analysis of mouse small intestine using 26778-1-AP

  mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 26778-1-AP (MYO6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of PC-3 using 26778-1-AP

  IP result of anti-MYO6(IP:26778-1-AP, 4ug; Detection:26778-1-AP 1:500) with PC-3 cells lysate 2000 ug.

• 

  IHC staining of human prostate cancer using 26778-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 26778-1-AP (MYO6 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human small intestine using 26778-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 26778-1-AP (MYO6 antibody) at dilution of 1:200 (under 40x lens).

• 

  IF Staining of mouse small intestine using 26778-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse small intestine tissue using 26778-1-AP (MYO6 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maus-Dünndarmgewebe
• Erfolgreiche IP: PC-3-Zellen
• Erfolgreiche Detektion in IHC: humanes Prostatakarzinomgewebe, humanes Dünndarmgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: Maus-Dünndarmgewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 2 publications below
• IF: See 2 publications below

Produktinformation

26778-1-AP bindet in WB, IP, IHC, IF, ELISA MYO6 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MYO6 fusion protein Ag24906
• Vollständiger Name: myosin VI
• Berechnetes Molekulargewicht: 1285 aa, 149 kDa
• Beobachtetes Molekulargewicht: 145-150 kDa
• GenBank-Zugangsnummer: BC146764
• Gene symbol: MYO6
• Gene ID (NCBI): 4646
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MYO6, an actin-based motor protein, is the only myosin known to move toward the minus end of actin filaments. MYO6 is highly expressed in the inner and outer hair cells of the ear, retina, and polarized epithelial cells such as kidney proximal tubule cells and intestinal enterocytes. And it participates in a wide range of biological processes within cells, including clathrin-mediated endocytosis, vesicular membrane traffic, polarized secretion, and autophagy (PMID: 23620821; PMID: 28591580). Previous studies showed that MYO6 is upregulated in various types of cancer, and it has been widely reported to contribute to tumor cell migration and metastasis. Some articles indicate that MYO6 is associated with prostate cancer, lung cancer, human colorectal cancer and gastric cancer (PMID: 29022908).

Publikationen

Species	Application	Title
human	WB	Nat Commun; Structure of Myosin VI/Tom1 complex reveals a cargo recognition mode of Myosin VI for tethering.; Authors - Shichen Hu
human	WB,IHC	Cell Signal; Elevated expression of myosin VI contributes to breast cancer progression via MAPK/ERK signaling pathway; Authors - Xiao-Juan Zhan ; KD Validated
mouse	WB,IHC,IF	Nat Commun; MYL3 protects chondrocytes from senescence by inhibiting clathrin-mediated endocytosis and activating of Notch signaling; Authors - He Cao
mouse	IF	Int J Toxicol; Copper Chaperone Atox1 Protected the Cochlea From Cisplatin by Regulating the Copper Transport Family and Cell Cycle; Authors - Xubo Chen

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Wojciech (Verified Customer) (04-20-2023)

We used this antibody to assess protein levels in human T cells. We got a good signal with 1:1000 dilution with observed molecular weight at ~150kDa.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: T cells

FH

Sara (Verified Customer) (10-25-2020)

B cells (mouse) in RIPA buffer. 10 µg of protein per lane were loaded on a 10% PAGE-SDS gel. Transfer is not optimised for high MW proteins, so I could get a better blot. Two bands are detected, one of 150 kDa and one > 200 kDa.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Mouse B cells from spleen

MYO6 Antibody Western Blot validation (1:1000 dilution) in Mouse B cells from spleen (Cat no:26778-1-AP)