MYO19 Polyklonaler Antikörper

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• KD/KO Validated

MYO19 Polyklonal Antikörper für IF, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Affe, human

Anwendung

WB, IF, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 23906-1-AP

Synonyme

MYO19, MYOHD1, myosin XIX

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Galerie der Validierungsdaten

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  WB analysis of HeLa using 23906-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 23906-1-AP (MYO19 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 23906-1-AP

  WB result of MYO19 antibody (23906-1-AP, 1:300) with si-Control and si-MYO19 transfected A549 cells.

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  WB analysis of COS-7 using 23906-1-AP

  COS-7 cells were subjected to SDS PAGE followed by western blot with 23906-1-AP (MYO19 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 23906-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 23906-1-AP (MYO19 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of A549 using 23906-1-AP

  Immunofluorescent analysis of A549 cells using 23906-1-AP (MYO19 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, A549-Zellen, COS-7-Zellen
• Erfolgreiche Detektion in IF: A549-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunfluoreszenz (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

23906-1-AP bindet in WB, IF, ELISA MYO19 und zeigt Reaktivität mit Affe, human

• Getestete Reaktivität: Affe, human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MYO19 fusion protein Ag20995
• Vollständiger Name: myosin XIX
• Berechnetes Molekulargewicht: 970 aa, 109 kDa
• Beobachtetes Molekulargewicht: 109 kDa
• GenBank-Zugangsnummer: BC008900
• Gene symbol: MYO19
• Gene ID (NCBI): 80179
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MYO19 is also named as MYOHD1. It is Actin-based motor molecule with ATPase activity that localizes to the mitochondrion outer membrane (PubMed:19932026, PubMed:23568824, PubMed:25447992) and required for mitochondrial inheritance during mitosis (PubMed:25447992). MYO19 can enable actin binding activity and be involved in regulation of cytokinesis and regulation of mitochondrial fission. MYO19 can be detected as about 109 kDa.