MYO10 Polyklonaler Antikörper

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• KD/KO Validated

MYO10 Polyklonal Antikörper für FC, IF, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

5

Kat-Nr. : 24565-1-AP

Synonyme

KIAA0799, MYO10, myosin X, Unconventional myosin 10

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Galerie der Validierungsdaten

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  WB analysis of mouse brain using 24565-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 24565-1-AP (MYO10 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 24565-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 24565-1-AP (MYO10 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IF Staining of SH-SY5Y using 24565-1-AP

  Immunofluorescent analysis of SH-SY5Y cells using 24565-1-AP (MYO10 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of RAW 264.7 using 24565-1-AP

  1X10^6 RAW 264.7 cells were stained with 0.2ug MYO10 antibody (24565-1-AP, red) and control antibody (blue). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500. Cells were fixed with 4% PFA and permeabilized with 0.1% Triton X-100.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, HeLa-Zellen
• Erfolgreiche Detektion in IF: SH-SY5Y-Zellen
• Erfolgreiche Detektion in FC: RAW 264.7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunfluoreszenz (IF): IF : 1:20-1:200
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Produktinformation

24565-1-AP bindet in WB, IHC, IF, FC, ELISA MYO10 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus, Zebrafisch
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MYO10 fusion protein Ag18882
• Vollständiger Name: myosin X
• Berechnetes Molekulargewicht: 2058 aa, 237 kDa
• Beobachtetes Molekulargewicht: 240-250 kDa
• GenBank-Zugangsnummer: BC137168
• Gene symbol: MYO10
• Gene ID (NCBI): 4651
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	IF	Sci Adv; MYO10 drives genomic instability and inflammation in cancer.; Authors - Franklin Mayca Pozo ; KO Validated
zebrafish	IHC	Elife; Retrograde Ret signaling controls sensory pioneer axon outgrowth.; Authors - Adam Tuttle
mouse	IF	PLoS One; NIMA-related kinase 1 (NEK1) regulates meiosis I spindle assembly by altering the balance between α-Adducin and Myosin X.; Authors - Miguel A Brieño-Enríquez
human	WB	Proteomics Clin Appl; Quantitative Proteome of Infant Stenotic Ureters Reveals Extracellular Matrix Organization and Oxidative Stress Dysregulation Underlying Ureteropelvic Junction Obstruction.; Authors - Yueyan Li
human	WB	Cytoskeleton (Hoboken); The myosin regulatory light chain Myl5 localizes to mitotic spindle poles and is required for proper cell division.; Authors - Ivan Ramirez