MYL9 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

MYL9 Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

8

Rezensionen (1)

Kat-Nr. : 15354-1-AP

Synonyme

20 kDa myosin light chain, LC20, MLC 2C, MLC2, MRLC1, MYL9, Myosin RLC, MYRL2

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Galerie der Validierungsdaten

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  WB analysis using 15354-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15354-1-AP (MYL9 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of Caco-2 using 15354-1-AP

  Caco-2 cells were subjected to SDS PAGE followed by western blot with 15354-1-AP (MYL9 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 15354-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15354-1-AP (MYL9 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 15354-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15354-1-AP (MYL9 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 15354-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15354-1-AP (MYL9 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 15354-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15354-1-AP (MYL9 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 15354-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15354-1-AP (MYL9 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of MCF-7 using 15354-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using MYL9 antibody (15354-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of MCF-7 using 15354-1-AP

  Immunofluorescent analysis of MCF-7 cells, using MYL9 antibody 15354-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

• 

  IF Staining of C2C12 using 15354-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed C2C12 cells using MYL9 antibody (15354-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maus-Kolongewebe, Caco-2-Zellen, Ratten-Kolongewebe
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe, humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: MCF-7-Zellen, C2C12-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:5000
• Immunhistochemie (IHC): IHC : 1:250-1:1000
• Immunfluoreszenz (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IHC: See 2 publications below
• IF: See 2 publications below
• IP: See 1 publications below

Produktinformation

15354-1-AP bindet in WB, IP, IHC, IF, ELISA MYL9 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MYL9 fusion protein Ag7600
• Vollständiger Name: myosin, light chain 9, regulatory
• Berechnetes Molekulargewicht: 20 kDa
• Beobachtetes Molekulargewicht: 20 kDa
• GenBank-Zugangsnummer: BC002648
• Gene symbol: MYL9
• Gene ID (NCBI): 10398
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Myosin regulatory light polypeptide 9 (MYL9), also known as MLC2, belongs to the myosin regulatory subunits. It plays an important role in regulation of both smooth muscle and nonmuscle cell contractile activity via its phosphorylation at Thr19 and Ser20. Implicated in cytokinesis, receptor capping, and cell locomotion (PMID:11942626, PMID:2526655). Some studies have demonstrated that MYL9 may play important roles in various human cancers. The expression and phosphorylation of MYL9 (Thr19/Ser20) may be increased in human breast (PMID: 22144583) and liver cancers (PMID: 18648664), while decreased in human colon (PMID: 22752057) and bladder cancers (PMID: 21139803). MYL9 was the only gene differentially expressed in the aged versus young injured arteries in the rat smooth muscle cell layers (PMID:22003410).

Publikationen

Species	Application	Title
		J Biol Chem; Brain-Derived Neurotrophic Factor regulates LYN kinase mediated Myosin Light Chain Kinase activation to modulate non-muscle myosin II activity in hippocampal neurons.; Authors - Xiao-Bing Li
mouse	IHC	Sci Rep; Proteomic profiling of concurrently isolated primary microvascular endothelial cells, pericytes, and vascular smooth muscle cells from adult mouse heart.; Authors - Zhiping Cao
human	WB	Front Med (Lausanne); Depiction of Aging-Based Molecular Phenotypes With Diverse Clinical Prognosis and Immunological Features in Gastric Cancer.; Authors - Fang He ; KD Validated
human	WB	Oncol Rep; Fenretinide inhibits the proliferation and migration of human liver cancer HepG2 cells by downregulating the activation of myosin light chain kinase through the p38‑MAPK signaling pathway.; Authors - Ling Zhang
mouse	IF	JCI Insight; Transient expansion and myofibroblast conversion of adipogenic lineage precursors mediate bone marrow repair after radiation.; Authors - Leilei Zhong
human	IF	Front Cell Dev Biol; Paracrine HB-EGF signaling reduce enhanced contractile and energetic state of activated decidual fibroblasts by rebalancing SRF-MRTF-TCF transcriptional axis; Authors - Junaid Afzal

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Irem (Verified Customer) (05-18-2022)

20 kDa band can be observed but there are other unspesific bands. Marker: Pageruler protein prestained marker

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Jurkat cells

MYL9 Antibody Western Blot validation (1:1000 dilution) in Jurkat cells (Cat no:15354-1-AP)