MYL5 Polyklonaler Antikörper

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• KD/KO Validated

MYL5 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Kat-Nr. : 14249-1-AP

Synonyme

MYL5, MyLC 2, MYLC2, Myosin light chain 5

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  WB analysis using 14249-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14249-1-AP (MYL5 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 14249-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 14249-1-AP (MYL5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human cervical cancer using 14249-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 14249-1-AP (MYL5 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cervical cancer using 14249-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 14249-1-AP (MYL5 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 14249-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 14249-1-AP (MYL5 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 14249-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 14249-1-AP (MYL5 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 14249-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 14249-1-AP (MYL5 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 14249-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 14249-1-AP (MYL5 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maus-Skelettmuskelgewebe, humanes Hirngewebe, Ratten-Skelettmuskelgewebe
• Erfolgreiche Detektion in IHC: humanes Zervixkarzinomgewebe, Mausnierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IF: See 1 publications below

Produktinformation

14249-1-AP bindet in WB, IF, IHC, ELISA MYL5 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MYL5 fusion protein Ag5522
• Vollständiger Name: myosin, light chain 5, regulatory
• Berechnetes Molekulargewicht: 20 kDa
• Beobachtetes Molekulargewicht: 20 kDa
• GenBank-Zugangsnummer: BC040050
• Gene symbol: MYL5
• Gene ID (NCBI): 4636
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB, IF	Cytoskeleton (Hoboken); The myosin regulatory light chain Myl5 localizes to mitotic spindle poles and is required for proper cell division.; Authors - Ivan Ramirez ; KD Validated