MYL4 Monoklonaler Antikörper

MYL4 Monoklonal Antikörper für FC, IF, IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

5

CloneNo.

2F5C1

Kat-Nr. : 67533-1-Ig

Synonyme

ALC1, AMLC, GT1, MLC1, MYL4, Myosin light chain 4, PRO1957

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Galerie der Validierungsdaten

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  WB analysis using 67533-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67533-1-Ig (MYL4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IHC staining of human heart using 67533-1-Ig

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 67533-1-Ig (MYL4 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse heart using 67533-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using MYL4 antibody (67533-1-Ig, Clone: 2F5C1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  FC experiment of C2C12 using 67533-1-Ig

  1X10^6 C2C12 cells were intracellularly stained with 0.4 ug Anti-Human MYL4 (67533-1-Ig, Clone:2F5C1) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Hausschwein-Herzgewebe, rat heart tissues, mouse heart tissues
• Erfolgreiche Detektion in IHC: humanes Herzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: Mausherzgewebe
• Erfolgreiche Detektion in FC: C2C12-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:150-1:600
• Immunfluoreszenz (IF): IF : 1:200-1:800
• Durchflusszytometrie (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IF: See 3 publications below

Produktinformation

67533-1-Ig bindet in WB, IHC, IF, FC, ELISA MYL4 und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hausschwein, Maus
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: MYL4 fusion protein Ag19244
• Vollständiger Name: myosin, light chain 4, alkali; atrial, embryonic
• Berechnetes Molekulargewicht: 197 aa, 22 kDa
• Beobachtetes Molekulargewicht: 22-25 kDa
• GenBank-Zugangsnummer: BC030228
• Gene symbol: MYL4
• Gene ID (NCBI): 4635
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Myosin light‐chain 4 (MYL4) encodes an essential myosin light chain that is expressed in embryonic whole heart and skeletal muscle. It is specifically expressed in human atria after birth. MYL4 is essential for atrial electrical, functional, and structural integrity, and MYL4 dysfunction can produce severe inherited atrial cardiomyopathy.

Publikationen

Species	Application	Title
human	IF	Front Cell Dev Biol; HiPS-Endothelial Cells Acquire Cardiac Endothelial Phenotype in Co-culture With hiPS-Cardiomyocytes.; Authors - Emmi Helle
mouse	WB,IF	Genes (Basel); Molecular Mechanism of MYL4 Regulation of Skeletal Muscle Development in Pigs; Authors - Xueli Xu ; KD Validated
pig	WB	Gene; Haplotypes within the regulatory region of MYL4 are associated with pig muscle fiber size; Authors - Shixiong Dong
mouse	WB	Cell Biosci; Motoneurons innervation determines the distinct gene expressions in multinucleated myofibers; Authors - Lei Bai
mouse	IF	Front Med; Distinct mononuclear diploid cardiac subpopulation with minimal cell-cell communications persists in embryonic and adult mammalian heart; Authors - Miaomiao Zhu

Verwandte Produkte

Flow Cytometry Perm Buffer (10X)

PF00011-C