Myosin Light Chain 2/MLC-2V Monoklonaler Antikörper

Myosin Light Chain 2/MLC-2V Monoklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Kaninchen, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

3

CloneNo.

5A1F1

Rezensionen (1)

Kat-Nr. : 60229-1-Ig

Synonyme

CMH10, MLC 2, MLC 2v, MLC2, MLC2v, MYL2, Myosin Light Chain 2, Myosin Light Chain 2/MLC-2V

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Galerie der Validierungsdaten

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  WB analysis using 60229-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60229-1-Ig (Myosin Light Chain 2/MLC-2V antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig heart using 60229-1-Ig

  pig heart tissue were subjected to SDS PAGE followed by western blot with 60229-1-Ig (Myosin Light Chain 2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat heart using 60229-1-Ig

  rat heart tissue were subjected to SDS PAGE followed by western blot with 60229-1-Ig (Myosin Light Chain 2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human heart using 60229-1-Ig

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 60229-1-Ig (Myosin Light Chain 2 antibody) at dilution of 1:500 (under 0x lens).

• 

  IHC staining of human heart using 60229-1-Ig

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 60229-1-Ig (Myosin Light Chain 2 antibody) at dilution of 1:500 (under 0x lens).

• 

  IHC staining of mouse skeletal muscle using 60229-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 60229-1-Ig (Myosin Light Chain 2/MLC-2V antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 60229-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 60229-1-Ig (Myosin Light Chain 2/MLC-2V antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of C2C12 using 60229-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed C2C12 cell using 60229-1-Ig (Myosin Light Chain 2 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of C2C12 using 60229-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed C2C12 cells using Myosin Light Chain 2/MLC-2V antibody (60229-1-Ig, Clone: 5A1F1 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Herzgewebe, Hausschwein-Herzgewebe, Kaninchenherzgewebe, Rattenherzgewebe
• Erfolgreiche Detektion in IHC: Mausherzgewebe, humanes Herzgewebe, Maus-Skelettmuskelgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: C2C12-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF): IF : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IF: See 2 publications below

Produktinformation

60229-1-Ig bindet in WB, IHC, IF, ELISA Myosin Light Chain 2/MLC-2V und zeigt Reaktivität mit Hausschwein, human, Kaninchen, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Kaninchen, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Myosin Light Chain 2/MLC-2V fusion protein Ag1356
• Vollständiger Name: myosin, light chain 2, regulatory, cardiac, slow
• Berechnetes Molekulargewicht: 19 kDa
• Beobachtetes Molekulargewicht: 18 kDa
• GenBank-Zugangsnummer: BC015821
• Gene symbol: MYL2
• Gene ID (NCBI): 4633
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MYL2, also named as MLC-2v and MLC-2, is ventricular/cardiac muscle isoform. Defects in MYL2 are the cause of cardiomyopathy familial hypertrophic type 10 (CMH10). Defects in MYL2 are the cause of cardiomyopathy familial hypertrophic with mid-left ventricular chamber type 2 (MVC2). MYL2 has been widely used as a marker of mature ventricular cardiomyocytes.

Publikationen

Species	Application	Title
human	IF	Stem Cells; Nitric Oxide-cGMP-PKG Pathway Acts on Orai1 to Inhibit the Hypertrophy of Human Embryonic Stem Cell-Derived Cardiomyocytes.; Authors - Y Wang
rat	WB	Int J Stem Cells; Synergistic Effect of Hydrogen and 5-Aza on Myogenic Differentiation through the p38 MAPK Signaling Pathway in Adipose-Derived Mesenchymal Stem Cells; Authors - Jingcheng Wang
human	IF	Stem Cell Rev Rep; MYOCD is Required for Cardiomyocyte-like Cells Induction from Human Urine Cells and Fibroblasts Through Remodeling Chromatin.; Authors - Xiangyu Zhang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Irem (Verified Customer) (04-19-2022)

Unfortunately, this antibody unspecifically binds to many proteins in Jurkat cells.

• Applications: Western Blot
• Primary Antibody Dilution: 1:800
• Cell Tissue Type: Jurkat cells

Myosin Light Chain 2/MLC-2V Antibody Western Blot validation (1:800 dilution) in Jurkat cells (Cat no:60229-1-Ig)