MYH14 Polyklonaler Antikörper

MYH14 Polyklonal Antikörper für FC, IF, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

12

Kat-Nr. : 20716-1-AP

Synonyme

MHC16, MYH14, myosin, Myosin 14, Myosin heavy chain 14, myosin, heavy chain 14, NMHC II C, Non muscle myosin heavy chain IIc, non-muscle II c

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Galerie der Validierungsdaten

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  WB analysis using 20716-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20716-1-AP (MYH14 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of human skeletal muscle using 20716-1-AP

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 20716-1-AP (MYH14 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse colon using 20716-1-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 20716-1-AP (MYH14 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse kidney using 20716-1-AP

  IP Result of anti-MYH14 (IP:20716-1-AP, 4ug; Detection:20716-1-AP 1:2000) with mouse kidney tissue lysate 4800ug.

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  IF Staining of HeLa using 20716-1-AP

  Immunofluorescent analysis of Hela cells, using MYH14 antibody 20716-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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  FC experiment of HT-29 using 20716-1-AP

  1X10^6 HT-29 cells were intracellularly stained with 0.2 ug Anti-Human MYH14 (20716-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with 0.1% TritonX-100.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: COLO 320-Zellen, HEK-293-Zellen, humanes Skelettmuskelgewebe, Maus-Kolongewebe, Mausnierengewebe, Rattennierengewebe
• Erfolgreiche IP: Mausnierengewebe
• Erfolgreiche Detektion in IF: HeLa-Zellen
• Erfolgreiche Detektion in FC: HT-29-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:5000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunfluoreszenz (IF): IF : 1:10-1:100
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 5 publications below
• IHC: See 2 publications below
• IF: See 8 publications below

Produktinformation

20716-1-AP bindet in WB, IP, IHC, IF, FC, ELISA MYH14 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: myosin, heavy chain 14
• Berechnetes Molekulargewicht: 228 kDa
• Beobachtetes Molekulargewicht: 228 kDa
• GenBank-Zugangsnummer: NM_024729
• Gene symbol: MYH14
• Gene ID (NCBI): 79784
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MYH14, also named as KIAA2034 and NMHC II-C, is cellular myosin that appears to play a role in cytokinesis, cell shape, and specialized functions such as secretion and capping. Defects in MYH14 are the cause of deafness autosomal dominant type 4 (DFNA4). The antibody is specific to MYH14.

Publikationen

Species	Application	Title
mouse	WB	Sci Adv; Promoting the activation of T cells with glycopolymer-modified dendritic cells by enhancing cell interactions.; Authors - Liyin Yu
human	WB	J Cell Biol; Cingulin and paracingulin tether myosins-2 to junctions to mechanoregulate the plasma membrane; Authors - Florian Rouaud
human	IHC	Clin Genet; Mutations of MYH14 Are Associated to Anorectal Malformations with Recto-perineal Fistulas in a Small Subset of Chinese Population.; Authors - Zhongxian Zhu
human	IF	Stem Cells Int; Pyridoxal-5'-Phosphate Promotes Immunomodulatory Function of Adipose-Derived Mesenchymal Stem Cells through Indoleamine 2,3-Dioxygenase-1 and TLR4/NF-κB Pathway.; Authors - Cong Li
mouse	IF	Mol Biol Cell; Nonmuscle myosin-2 contractility-dependent actin turnover limits the length of epithelial microvilli.; Authors - Colbie R Chinowsky
mouse	WB,IF	Life (Basel); Mass Spectrometric Profiling of Extraocular Muscle and Proteomic Adaptations in the mdx-4cv Model of Duchenne Muscular Dystrophy.; Authors - Stephen Gargan