MYH10 Monoklonaler Antikörper

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• KD/KO Validated

MYH10 Monoklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

CloneNo.

2H8G7

Kat-Nr. : 67243-1-Ig

Synonyme

MYH10, Myosin 10, Myosin heavy chain 10

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Galerie der Validierungsdaten

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  WB analysis of mouse brain using 67243-1-Ig

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 67243-1-Ig (MYH10 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 67243-1-Ig

  WB result of MYH10 antibody (67243-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MYH10 transfected HEK-293 cells.

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  WB analysis of rat brain using 67243-1-Ig

  rat brain tissue were subjected to SDS PAGE followed by western blot with 67243-1-Ig (MYH10 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat cerebellum using 67243-1-Ig

  rat cerebellum tissue were subjected to SDS PAGE followed by western blot with 67243-1-Ig (MYH10 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse cerebellum using 67243-1-Ig

  mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 67243-1-Ig (MYH10 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse cerebellum using 67243-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 67243-1-Ig (MYH10 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse cerebellum using 67243-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 67243-1-Ig (MYH10 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse cerebellum using 67243-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using MYH10 antibody (67243-1-Ig, Clone: 2H8G7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, Maus-Cerebellum-Gewebe, Ratten-Cerebellum-Gewebe, Rattenhirngewebe
• Erfolgreiche Detektion in IHC: Maus-Cerebellum-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: Maus-Cerebellum-Gewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunhistochemie (IHC): IHC : 1:150-1:600
• Immunfluoreszenz (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

67243-1-Ig bindet in WB, IHC, IF, ELISA MYH10 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: MYH10 fusion protein Ag17069
• Vollständiger Name: myosin, heavy chain 10, non-muscle
• Berechnetes Molekulargewicht: 1985 aa, 230 kDa
• Beobachtetes Molekulargewicht: 200-230 kDa
• GenBank-Zugangsnummer: BC150634
• Gene symbol: MYH10
• Gene ID (NCBI): 4628
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MYH10 (non-muscle II-b, NM IIB) is a member of non-muscle myosin II which plays fundamental roles in the maintenance of cell morphology, cell adhesion, and migration, as well as cell division. MYH10 is mainly present in nerve cells, megakaryocytes, and other non-muscle cells. It has been reported to mediate centrosome reorientation during cell migration and contribute to ciliogenesis. Overexpression of MYH10 has been observed in breast cancer.