B-Myb Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

B-Myb Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IP, IHC, IF, ChIP, ELISA

Konjugation

Unkonjugiert

Publikationen

12

Kat-Nr. : 18896-1-AP

Synonyme

B MYB, BMYB, B-Myb, Myb like protein 2, Myb related protein B, MYBL2

---

Galerie der Validierungsdaten

• 

  WB analysis of HeLa using 18896-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 18896-1-AP (B-Myb antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 18896-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 18896-1-AP (B-Myb antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 18896-1-AP

  IP Result of anti-B-Myb (IP:18896-1-AP, 4ug; Detection:18896-1-AP 1:300) with HeLa cells lysate 1520ug.

• 

  IHC staining of human prostate cancer using 18896-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 18896-1-AP (B-Myb antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 18896-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 18896-1-AP (B-Myb antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Prostatakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 4 publications below
• WB: See 11 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• IP: See 1 publications below
• ChIP: See 2 publications below

Produktinformation

18896-1-AP bindet in WB, IP, IHC, IF, ChIP, ELISA B-Myb und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: B-Myb fusion protein Ag13525
• Vollständiger Name: v-myb myeloblastosis viral oncogene homolog (avian)-like 2
• Berechnetes Molekulargewicht: 79 kDa
• Beobachtetes Molekulargewicht: 90 kDa
• GenBank-Zugangsnummer: BC007585
• Gene symbol: MYBL2
• Gene ID (NCBI): 4605
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MYBL2, also named as BMYB, is a 700 amino acid protein, which is phosphorylated by cyclin A/CDK2 during S-phase. MYBL2 is a transcription factor involved in the regulation of cell survival, proliferation, and differentiation. The calculated molecular weight of MYBL2 is 79 kDa, but modified MYBL2 is about 90 kDa (PMID: 11418655).

Publikationen

Species	Application	Title
human	WB	Oxid Med Cell Longev; LncRNA-MALAT1 Regulates Cancer Glucose Metabolism in Prostate Cancer via MYBL2/mTOR Axis.; Authors - Xiangyu Mu
human,mouse	WB	J Virol; Minute Virus of Mice (MVM) inhibits transcription of the cyclin B1 gene during infection.; Authors - Matthew S Fuller
human	WB,IHC,IP	Cancer Gene Ther; A targetable MYBL2-ATAD2 axis governs cell proliferation in ovarian cancer; Authors - Qun Liu ; KO Validated
mouse	WB	Mol Cells; Sertad1 Induces Neurological Injury after Ischemic Stroke via the CDK4/p-Rb Pathway.; Authors - Jianxiong Li
human	WB	Mediators Inflamm; Knockdown MTDH Inhibits Glioma Proliferation and Migration and Promotes Apoptosis by Downregulating MYBL2; Authors - Junqi Fu
human	WB	Cancer Biol Ther; The differences of regulatory networks between papillary and anaplastic thyroid carcinoma: an integrative transcriptomics study.; Authors - Zongfu Pan