MYBBP1A Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

MYBBP1A Polyklonal Antikörper für FC, IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IP, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Kat-Nr. : 14524-1-AP

Synonyme

MYB binding protein (P160) 1a, Myb binding protein 1A, MYBBP1A, P160, PAP2

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Galerie der Validierungsdaten

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  WB analysis using 14524-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14524-1-AP (MYBBP1A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 14524-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 14524-1-AP (MYBBP1A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 14524-1-AP

  IP Result of anti-MYBBP1A (IP:14524-1-AP, 4ug; Detection:14524-1-AP 1:500) with HEK-293 cells lysate 2800ug.

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  IHC staining of human kidney using 14524-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 14524-1-AP (MYBBP1A antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human kidney using 14524-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 14524-1-AP (MYBBP1A antibody) at dilution of 1:100 (under 10x lens).

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  IF Staining of HepG2 using 14524-1-AP

  Immunofluorescent analysis of HepG2 cells, using MYBBP1A antibody 14524-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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  FC experiment of HepG2 using 14524-1-AP

  1X10^6 HepG2 cells were stained with 0.2ug MYBBP1A antibody (14524-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HEK-293-Zellen
• Erfolgreiche IP: HEK-293-Zellen
• Erfolgreiche Detektion in IHC: humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen
• Erfolgreiche Detektion in FC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:20-1:200
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IF: See 1 publications below

Produktinformation

14524-1-AP bindet in WB, IP, IHC, IF, FC, ELISA MYBBP1A und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MYBBP1A fusion protein Ag6008
• Vollständiger Name: MYB binding protein (P160) 1a
• Berechnetes Molekulargewicht: 149 kDa
• Beobachtetes Molekulargewicht: 150-160 kDa
• GenBank-Zugangsnummer: BC050546
• Gene symbol: MYBBP1A
• Gene ID (NCBI): 10514
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The protooncogene MYB is predominantly expressed in immature hemopoietic cells where it has an essential role in hemopoietic cell proliferation and differentiation. Oncogenically activated forms of MYB is generally N- and/or C-terminal truncations of the normal MYB protein. Removal of the C terminus of MYB disrupts or deletes a region termed the negative regulatory domain (NRD), resulting in an increase in DNA binding, transactivation, and transformation by MYB. One feature of the NRD is a leucine zipper-like motif [PMID: 8302594]. Murine Myb-binding protein-1a (MYBBP1A), originally called P160, was identified by its ability to interact specifically with Myb via this leucine zipper-like motif. MYBBP1A modulates MYB activity upon binding to the MYB NRD [PMID: 10644447, 9447996].

Publikationen

Species	Application	Title
mouse	WB	Cell Rep; A fetal tumor suppressor axis abrogates MLL-fusion-driven acute myeloid leukemia; Authors - Mohamed Eldeeb
mouse	WB	Sci Adv; In vivo protein turnover rates in varying oxygen tensions nominate MYBBP1A as a mediator of the hyperoxia response; Authors - Xuewen Chen
human	WB,IF	Cancers (Basel); Loss of MYBBP1A Induces Cancer Stem Cell Activity in Renal Cancer.; Authors - Blanca Felipe-Abrio ; KD Validated
human	WB	Mol Oncol; c-MYB- and PGC1a-dependent metabolic switch induced by MYBBP1A loss in renal cancer.; Authors - Blanca Felipe-Abrio