MYB/c-Myb Monoklonaler Antikörper

MYB/c-Myb Monoklonal Antikörper für FC, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, FC, ELISA

Konjugation

Unkonjugiert

CloneNo.

2C4A5

Kat-Nr. : 68542-1-Ig

Synonyme

c myb, c myb_CDS, Cmyb, efg, MYB, Proto oncogene c Myb, Transcriptional activator Myb

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Galerie der Validierungsdaten

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  WB analysis using 68542-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68542-1-Ig (MYB/c-Myb antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  FC experiment of Jurkat using 68542-1-Ig

  1X10^6 Jurkat cells were intracellularly stained with 0.4 ug Anti-Human MYB/c-Myb (68542-1-Ig, Clone:2C4A5) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, MOLT-4-Zellen
• Erfolgreiche Detektion in FC: Jurkat-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Durchflusszytometrie (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

68542-1-Ig bindet in WB, FC, ELISA MYB/c-Myb und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: MYB/c-Myb fusion protein Ag12021
• Vollständiger Name: v-myb myeloblastosis viral oncogene homolog (avian)
• Berechnetes Molekulargewicht: 640 aa, 72 kDa
• Beobachtetes Molekulargewicht: 40-50 kDa, 72-80 kDa
• GenBank-Zugangsnummer: BC064955
• Gene symbol: MYB
• Gene ID (NCBI): 4602
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The MYB protein is a transcription factor that plays a key role in regulating the proliferation/differentiation of progenitor cells in bone marrow, colonic crypts, and neurogenic niches [PMID:18574464]. It is involved in the regulation of epithelial-to-mesenchymal transition (EMT) and invasion in neuroblastoma, colon carcinoma, and embryonic kidney cells through the upregulation of the transcription repressor Slug [PMID:20622260]. It also a DNA-binding protein that specifically recognize the sequence 5'-YAAC[GT]G-3', and plays an important role in the control of proliferation and differentiation of hematopoietic progenitor cells. MYB exists some isoforms with MV 85, 75, 72, 68, 63 and 40 kDa.