MYB/c-Myb Polyklonaler Antikörper

MYB/c-Myb Polyklonal Antikörper für IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IP, IF, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

9

Kat-Nr. : 17800-1-AP

Synonyme

c myb_CDS, Cmyb, c-Myb, efg, MYB, Proto oncogene c Myb, Transcriptional activator Myb

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Galerie der Validierungsdaten

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  WB analysis using 17800-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17800-1-AP (c-Myb antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 17800-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17800-1-AP (c-Myb antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 17800-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 17800-1-AP (c-Myb antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of HL-60 using 17800-1-AP

  IP Result of anti-c-Myb (IP:17800-1-AP, 4ug; Detection:17800-1-AP 1:300) with HL-60 cells lysate 4000ug.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HL-60-Zellen, Jurkat-Zellen, MOLT-4-Zellen
• Erfolgreiche IP: HL-60-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 9 publications below
• IF: See 1 publications below
• CoIP: See 2 publications below

Produktinformation

17800-1-AP bindet in WB, IP, IF, CoIP, ELISA MYB/c-Myb und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MYB/c-Myb fusion protein Ag12021
• Vollständiger Name: v-myb myeloblastosis viral oncogene homolog (avian)
• Berechnetes Molekulargewicht: 640 aa, 72 kDa
• Beobachtetes Molekulargewicht: 40-50 kDa, 72-80 kDa
• GenBank-Zugangsnummer: BC064955
• Gene symbol: MYB
• Gene ID (NCBI): 4602
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The MYB protein is a transcription factor that plays a key role in regulating the proliferation/differentiation of progenitor cells in bone marrow, colonic crypts, and neurogenic niches [PMID:18574464]. It is involved in the regulation of epithelial-to-mesenchymal transition (EMT) and invasion in neuroblastoma, colon carcinoma, and embryonic kidney cells through the upregulation of the transcription repressor Slug [PMID:20622260]. It also a DNA-binding protein that specifically recognize the sequence 5'-YAAC[GT]G-3', and plays an important role in the control of proliferation and differentiation of hematopoietic progenitor cells. MYB exists some isoforms with MV 85, 75, 72, 68, 63 and 40 kDa.

Publikationen

Species	Application	Title
human	WB	Nat Commun; NLRP3 phosphorylation in its LRR domain critically regulates inflammasome assembly.; Authors - Tingting Niu
mouse	WB	Nat Commun; Tcf1 and Lef1 provide constant supervision to mature CD8+ T cell identity and function by organizing genomic architecture.; Authors - Qiang Shan
human	WB,CoIP	RNA Biol; SND1 acts as an anti-apoptotic factor via regulating the expression of lncRNA UCA1 in hepatocellular carcinoma.; Authors - Xiaoteng Cui
human	IF,WB	Front Endocrinol (Lausanne); KRT17 from skin cells with high glucose stimulation promotes keratinocytes proliferation and migration; Authors - Peng Zhou
human	WB	Front Med (Lausanne); Lidocaine and Bupivacaine Downregulate MYB and DANCR lncRNA by Upregulating miR-187-5p in MCF-7 Cells.; Authors - Chiao-Yi Lin
mouse	WB	Cancer Genomics Proteomics; Myb Repression Mediates Stat5b-knockdown-induced Apoptosis and Inhibits Proliferation of Glioblastoma Stem Cells; Authors - Chiami Moyama