MUC8 Polyklonaler Antikörper

MUC8 Polyklonal Antikörper für IF, IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF

Konjugation

Unkonjugiert

Publikationen

1

Kat-Nr. : 55489-1-AP

Synonyme

MUC8, mucin 8

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Galerie der Validierungsdaten

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  WB analysis of A549 using 55489-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 55489-1-AP (MUC8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 55489-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 55489-1-AP (MUC8 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human lung cancer using 55489-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 55489-1-AP (MUC8 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung cancer using 55489-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 55489-1-AP (MUC8 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human lung cancer using 55489-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 55489-1-AP (MUC8 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung cancer using 55489-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 55489-1-AP (MUC8 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human lung using 55489-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 55489-1-AP (MUC8 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung using 55489-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 55489-1-AP (MUC8 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human lung cancer using 55489-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 55489-1-AP (MUC8 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung cancer using 55489-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 55489-1-AP (MUC8 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of A549 using 55489-1-AP

  Immunofluorescent analysis of A549 cells using 55489-1-AP (MUC8 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe, humanes Lungengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: A549-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IHC: See 1 publications below

Produktinformation

55489-1-AP bindet in WB, IHC, IF MUC8 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: mucin 8
• Berechnetes Molekulargewicht: 33 kDa
• Beobachtetes Molekulargewicht: 33-38 kDa
• GenBank-Zugangsnummer: U14383
• Gene symbol: MUC8
• Gene ID (NCBI): 4590
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20℃ lagern. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Mucin glycoproteins (Mucins) are major constituents of the glycocalyx that covers mucosal epithelium. MUC8 is one of the major mucins in the ethmoid mucosa and is upregulated by chronic inflammation. TNFa, IL-1b and a combination of both can significantly increase Mucin 8 mRNA levels, suggesting that a mixture of inflammatory mediators can synergistically increase secretion of mucin in human nasal epithelium. This antibody is specific to MUC8.

Publikationen

Species	Application	Title
human	IHC,WB	Diagnostics (Basel); The Translational Role of MUC8 in Salivary Glands: A Potential Biomarker for Salivary Stone Disease?; Authors - Martin Schicht