MUC13 Polyklonaler Antikörper

MUC13 Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 29832-1-AP

Synonyme

DRCC1, FLJ20063, MUC 13, MUC13, Mucin 13, RECC

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Galerie der Validierungsdaten

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  WB analysis using 29832-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29832-1-AP (MUC13 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 29832-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 29832-1-AP (MUC13 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 29832-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 29832-1-AP (MUC13 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 29832-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 29832-1-AP (MUC13 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 29832-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 29832-1-AP (MUC13 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 29832-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 29832-1-AP (MUC13 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 29832-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 29832-1-AP (MUC13 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HCT 116-Zellen, HEK-293-Zellen, HT-29-Zellen, Mausnierengewebe, Rattennierengewebe, SW480-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe, humanes Kolongewebe, humanes Magenkrebsgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunhistochemie (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

29832-1-AP bindet in WB, IHC, ELISA MUC13 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MUC13 fusion protein Ag31090
• Vollständiger Name: mucin 13, cell surface associated
• Berechnetes Molekulargewicht: 55 kDa
• Beobachtetes Molekulargewicht: 70-120 kDa
• GenBank-Zugangsnummer: NM_033049
• Gene symbol: MUC13
• Gene ID (NCBI): 56667
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Mucins (MUC) are highly glycosylated proteins that are abundantly expressed in mammalian epithelial cells. Mucin 13 (MUC13) is a member of the transmembrane glycoprotein mucins which is normally expressed in the large intestine, trachea, kidney, small intestine, and gastric epithelium (PMID: 22027689). MUC13 is involved in the tumorigenesis of multiple malignancies, including pancreatic cancer, colorectal cancer, renal cancer and lung cancer (PMID: 22027689; PMID: 31427737; PMID: 28205224; PMID: 34966453).