MTX1 Polyklonaler Antikörper

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• KD/KO Validated

MTX1 Polyklonal Antikörper für IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IP, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 15529-1-AP

Synonyme

metaxin 1, MTX, MTX1, MTXN

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Galerie der Validierungsdaten

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  WB analysis using 15529-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15529-1-AP (MTX1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis using 15529-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15529-1-AP (MTX1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IP experiment of MCF-7 using 15529-1-AP

  IP Result of anti-MTX1 (IP:15529-1-AP, 4ug; Detection:15529-1-AP 1:700) with MCF-7 cells lysate 1280ug.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, A549-Zellen, HeLa-Zellen, Jurkat-Zellen
• Erfolgreiche IP: MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 3 publications below
• IP: See 1 publications below

Produktinformation

15529-1-AP bindet in WB, IP, ELISA MTX1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MTX1 fusion protein Ag7685
• Vollständiger Name: metaxin 1
• Berechnetes Molekulargewicht: 51 kDa
• Beobachtetes Molekulargewicht: 35 kDa
• GenBank-Zugangsnummer: BC001906
• Gene symbol: MTX1
• Gene ID (NCBI): 4580
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

METAXINs are proteins located in the outer membrane of the mammalian mitochondrion. METAXIN 1 was characterized firstly and was found to play a role in the import of mitochondrial precursor proteins. It may also play a role in the assembly of b-barrel proteins into the outer membrane METAXIN1 was reported to be essential for survival of the postimplantation mouse embryo. METAXIN 1 has multiple transcripts. Several isoforms of METAXIN 1 exist due to the alternative splicing, with the predicted MW of 51, 48 and 36 kDa, respectively. Metaxin 1 interacts with metaxin 2, a novel related protein associated with the mammalian mitochondrial outer membrane and form complex in the mitochondrion outer membrane.

Publikationen

Species	Application	Title
human	WB,IP	Int J Biol Sci; Genome-scale CRISPRa screening identifies MTX1 as a contributor for sorafenib resistance in hepatocellular carcinoma by augmenting autophagy.; Authors - Li Li ; KD Validated
mouse	WB	Hepatology; Loss of Sam50 in hepatocytes induces cardiolipin-dependent mitochondrial membrane remodeling to trigger mtDNA release and liver injury; Authors - Li Chen
human	WB	J Cell Sci; Myo19 tethers mitochondria to endoplasmic reticulum-associated actin to promote mitochondrial fission; Authors - Stephen M Coscia ; KD Validated