MTP18 Polyklonaler Antikörper

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• KD/KO Validated

MTP18 Polyklonal Antikörper für IHC, IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

5

Kat-Nr. : 14257-1-AP

Synonyme

HSPC242, Mitochondrial 18 kDa protein, mitochondrial protein 18 kDa, MTFP1, MTP18, My022

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Galerie der Validierungsdaten

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  WB analysis of mouse skeletal muscle using 14257-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 14257-1-AP (MTP18 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 14257-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 14257-1-AP (MTP18 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse skeletal muscle using 14257-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 14257-1-AP (MTP18 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse heart using 14257-1-AP

  IP Result of anti-MTP18 (IP:14257-1-AP, 4ug; Detection:14257-1-AP 1:500) with mouse heart tissue lysate 4000ug.

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  IHC staining of human heart using 14257-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 14257-1-AP (MTP18 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human heart using 14257-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 14257-1-AP (MTP18 Antibody) at dilution of 1:200 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maus-Skelettmuskelgewebe, Mausherzgewebe
• Erfolgreiche IP: Mausherzgewebe
• Erfolgreiche Detektion in IHC: humanes Herzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:100-1:400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IHC: See 1 publications below

Produktinformation

14257-1-AP bindet in WB, IP, IHC, ELISA MTP18 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MTP18 fusion protein Ag5530
• Vollständiger Name: mitochondrial protein 18 kDa
• Berechnetes Molekulargewicht: 18 kDa
• Beobachtetes Molekulargewicht: 16-18 kDa
• GenBank-Zugangsnummer: BC038831
• Gene symbol: MTP18
• Gene ID (NCBI): 51537
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	J Cell Biol; Drp1-dependent mitochondrial fission via MiD49/51 is essential for apoptotic cristae remodeling.; Authors - Hidenori Otera
human	IHC	Cell Death Dis; MTP18 overexpression contributes to tumor growth and metastasis and associates with poor survival in hepatocellular carcinoma.; Authors - Yu Zhang
human	WB	Cell Biol Int; MTFP1 overexpression promotes the growth of oral squamous cell carcinoma by inducing ROS production.; Authors - Tingying Xiao ; KD Validated
mouse	WB	Front Cell Neurosci; Reduced mitochondrial size in hippocampus and psychiatric behavioral changes in the mutant mice with homologous mutation of Timm8a1-I23fs49X; Authors - Niemtiah Ouattara
mouse	WB	J Transl Med; TREM-1 triggers necroptosis of macrophages through mTOR-dependent mitochondrial fission during acute lung injury; Authors - Wen-Jing Zhong