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mTOR Monoklonaler Antikörper
mTOR Monoklonal Antikörper für IF, IHC, IP, WB, ELISA
Wirt / Isotyp
Maus / IgG2a
Getestete Reaktivität
human, Maus, Ratte und mehr (3)
Anwendung
WB, PLA, IP, IHC, IF, ELISA
Konjugation
Unkonjugiert
CloneNo.
1G11A3
Kat-Nr. : 66888-1-Ig
Synonyme
Galerie der Validierungsdaten
Geprüfte Anwendungen
Erfolgreiche Detektion in WB | LNCaP-Zellen, HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen, Jurkat-Zellen, K-562-Zellen, Maushirngewebe, NCI-H1299-Zellen, NIH/3T3-Zellen, Rattenhirngewebe, ROS1728-Zellen, THP-1-Zellen |
Erfolgreiche IP | HeLa-Zellen |
Erfolgreiche Detektion in IHC | humanes Kolonkarzinomgewebe, humanes Leberkarzinomgewebe, humanes Mammakarzinomgewebe Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen. |
Erfolgreiche Detektion in IF | HepG2-Zellen, HeLa-Zellen |
Empfohlene Verdünnung
Anwendung | Verdünnung |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunpräzipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunhistochemie (IHC) | IHC : 1:1000-1:4000 |
Immunfluoreszenz (IF) | IF : 1:400-1:1600 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, check data in validation data gallery |
Veröffentlichte Anwendungen
WB | See 241 publications below |
IHC | See 7 publications below |
IF | See 6 publications below |
IP | See 4 publications below |
Produktinformation
66888-1-Ig bindet in WB, PLA, IP, IHC, IF, ELISA mTOR und zeigt Reaktivität mit human, Maus, Ratten
Getestete Reaktivität | human, Maus, Ratte |
In Publikationen genannte Reaktivität | human, hamster, Hausschwein, Maus, Ratte, Ziege |
Wirt / Isotyp | Maus / IgG2a |
Klonalität | Monoklonal |
Typ | Antikörper |
Immunogen | mTOR fusion protein Ag28395 |
Vollständiger Name | FK506 binding protein 12-rapamycin associated protein 1 |
Berechnetes Molekulargewicht | 289 kDa |
Beobachtetes Molekulargewicht | 250-289 kDa |
GenBank-Zugangsnummer | NM_004958 |
Gene symbol | FRAP1 |
Gene ID (NCBI) | 2475 |
Konjugation | Unkonjugiert |
Form | Liquid |
Reinigungsmethode | Protein-A-Reinigung |
Lagerungspuffer | PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3. |
Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
MTOR, also named as FRAP1, FRAP, FRAP2 and RAPT1, belongs to the PI3/PI4-kinase family. MTOR is a Ser/Thr protein kinase that functions as an ATP and amino acid sensor to balance nutrient availability and cell growth. MTOR is kinase subunit of both mTORC1 and mTORC2, which regulate cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino-acids. mTORC2 is also activated by growth factors, but seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. MTOR has a calculated molecular mass of 289 kDa, and always can be detected at about 250 kDa due to some modifications (PMID: 14578359).
Protokolle
Produktspezifische Protokolle | |
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WB protocol for mTOR antibody 66888-1-Ig | Protokoll herunterladen |
IHC protocol for mTOR antibody 66888-1-Ig | Protokoll herunterladen |
IF protocol for mTOR antibody 66888-1-Ig | Protokoll herunterladen |
IP protocol for mTOR antibody 66888-1-Ig | Protokoll herunterladen |
Standard-Protokolle | |
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Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |
Publikationen
Species | Application | Title |
---|---|---|
Gastroenterology Nucleolar HEATR1 upregulated by mTORC1 signaling promotes hepatocellular carcinoma growth by dominating ribosome biogenesis and proteome homeostasis | ||
Mol Cell Lysosomal cyst(e)ine storage potentiates tolerance to oxidative stress in cancer cells | ||
Mol Cancer circEXOC6B interacting with RRAGB, an mTORC1 activator, inhibits the progression of colorectal cancer by antagonizing the HIF1A-RRAGB-mTORC1 positive feedback loop. | ||
Autophagy Periplocin suppresses the growth of colorectal cancer cells by triggering LGALS3 (galectin 3)-mediated lysophagy | ||
Nat Commun Location-specific inhibition of Akt reveals regulation of mTORC1 activity in the nucleus. | ||
J Clin Invest Up-regulated YB-1 protein promotes glioblastoma growth through an YB-1/CCT4/mLST8/mTOR pathway. |
Rezensionen
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Sun (Verified Customer) (01-05-2023) | strong band showed up around 200kDa region in HEK293T cells.
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FH Macarena (Verified Customer) (10-07-2022) | excellent band. Very concentrated.
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FH Rachel (Verified Customer) (05-24-2021) | Lovely antibody, very clean good siganl Cell lysates were run on JESS Simple Western
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