MTHFD1L Monoklonaler Antikörper

MTHFD1L Monoklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human, Kaninchen, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

CloneNo.

1G1F2

Kat-Nr. : 68321-1-Ig

Synonyme

dJ292B18.2, DKFZp586G1517, FLJ21145, FTHFSDC1, MTC1THFS, MTHFD1L

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Galerie der Validierungsdaten

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  WB analysis using 68321-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68321-1-Ig (MTHFD1L antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control.

• 

  WB analysis using 68321-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68321-1-Ig (MTHFD1L antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control.

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  IHC staining of mouse liver using 68321-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 68321-1-Ig (MTHFD1L antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse liver using 68321-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 68321-1-Ig (MTHFD1L antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: U2OS-Zellen, HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen, Kaninchenhodengewebe, LNCaP-Zellen, Rattenhodengewebe
• Erfolgreiche Detektion in IHC: Mauslebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

68321-1-Ig bindet in WB, IHC, ELISA MTHFD1L und zeigt Reaktivität mit human, Kaninchen, Maus, Ratten

• Getestete Reaktivität: human, Kaninchen, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: MTHFD1L fusion protein Ag9061
• Vollständiger Name: methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1-like
• Berechnetes Molekulargewicht: 978 aa, 106 kDa
• Beobachtetes Molekulargewicht: 106 kDa
• GenBank-Zugangsnummer: BC017477
• Gene symbol: MTHFD1L
• Gene ID (NCBI): 25902
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MTHFD1L(Monofunctional C1-tetrahydrofolate synthase, mitochondrial) is also named as FTHFSDC1(Formyltetrahydrofolate synthetase). MTHFD1L enzyme is present in mitochondria from normal embryonic tissues and embryonic fibroblast cell lines, and embryonic mitochondria possess the ability to synthesize formate from glycine. It catalyzes the final step in the mitochondrial conversion of 1-C units to formate in embryos. Moreover, MTHFD1L levels were substantially higher in embryonic mitochondria than in adult liver mitochondria and embryonic mitochondria exhibited greater formate production(PMID:19948730). It has 2 isoforms produced by alternative splicing.