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MTHFD1 Monoklonaler Antikörper

MTHFD1 Monoklonal Antikörper für IF

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

IF

Konjugation

CoraLite®555 Fluorescent Dye

CloneNo.

2B1G3

Kat-Nr. : CL555-67670

Synonyme

MTHFC, MTHFD, MTHFD1

Galerie der Validierungsdaten

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  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using CoraLite®555-conjugated MTHFD1 Monoclonal antibody (CL555-67670)

    IF Staining of HEK-293 using CL555-67670

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using CoraLite®555 MTHFD1 antibody (CL555-67670, Clone: 2B1G3 ) at dilution of 1:200.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using CoraLite®555-conjugated MTHFD1 Monoclonal antibody (CL555-67670)

    IF Staining of HEK-293 using CL555-67670

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using CoraLite®555 MTHFD1 antibody (CL555-67670, Clone: 2B1G3 ) at dilution of 1:200.

Geprüfte Anwendungen

Erfolgreiche Detektion in IFHEK-293-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Immunfluoreszenz (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

CL555-67670 bindet in IF MTHFD1 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
Wirt / Isotyp Maus / IgG1
Klonalität Monoklonal
Typ Antikörper
Immunogen MTHFD1 fusion protein Ag24553
Vollständiger Name methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1, methenyltetrahydrofolate cyclohydrolase, formyltetrahydrofolate synthetase
Berechnetes Molekulargewicht 101 kDa
Beobachtetes Molekulargewicht 101 kDa
GenBank-ZugangsnummerBC009806
Gene symbol MTHFD1
Gene ID (NCBI) 4522
Konjugation CoraLite®555 Fluorescent Dye
Excitation/Emission maxima wavelengths557 nm / 570 nm
Form Liquid
Reinigungsmethode Protein-G-Reinigung
Lagerungspuffer BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
LagerungsbedingungenBei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MTHFD1 is a trifunctional enzyme involved in the pathway tetrahydrofolate interconversion, which is part of One-carbon metabolism. MTHFD1 is associated with HCC and CRC progression. It has been reported that MTHFD-deficient patient fibroblasts exhibit enriched MTHFD1 in the nucleus which is critical for de novo dTMP synthesis.(PMID: 31133746, 30997850, 31421459, 25548164)

Protokolle

Produktspezifische Protokolle
IF protocol for CL555 MTHFD1 antibody CL555-67670Protokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen