MTF2 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

MTF2 Polyklonal Antikörper für IF, IHC, IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ChIP, ELISA

Konjugation

Unkonjugiert

Publikationen

25

Kat-Nr. : 16208-1-AP

Synonyme

hPCl2, M96, MTF2, PCL2, Polycomb like protein 2, RP5 976O13.1

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Galerie der Validierungsdaten

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  WB analysis of HepG2 using 16208-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16208-1-AP (MTF2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis using 16208-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 16208-1-AP (MTF2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse ES cells and 293 cells using 16208-1-AP

  WB result of anti-MTF2 (16208-1-AP) from Kevin Corbit.

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  IP experiment of mouse skeletal muscle using 16208-1-AP

  IP Result of anti-MTF2 (IP:16208-1-AP, 4ug; Detection:16208-1-AP 1:300) with mouse skeletal muscle tissue lysate 4000ug.

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  IHC staining of human brain using 16208-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 16208-1-AP (MTF2 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human brain using 16208-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 16208-1-AP (MTF2 antibody) at dilution of 1:50 (under 10x lens).

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  IF Staining of mouse ES cells using 16208-1-AP

  IF result of mouse ES cell From Kevin Corbit.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, Jurkat-Zellen, Maus-ES-Zellen und -293-Zellen
• Erfolgreiche IP: Maus-Skelettmuskelgewebe
• Erfolgreiche Detektion in IHC: humanes Hirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: Maus-ES-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:50-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 20 publications below
• IHC: See 2 publications below
• IF: See 1 publications below
• IP: See 1 publications below
• ChIP: See 5 publications below

Produktinformation

16208-1-AP bindet in WB, IP, IHC, IF, ChIP, ELISA MTF2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MTF2 fusion protein Ag9182
• Vollständiger Name: metal response element binding transcription factor 2
• Berechnetes Molekulargewicht: 536 aa, 61 kDa
• Beobachtetes Molekulargewicht: 67 kDa, 60 kDa, 60-61 kDa
• GenBank-Zugangsnummer: BC010013
• Gene symbol: MTF2
• Gene ID (NCBI): 22823
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MTF2, also named as Metal-response element-binding transcription factor 2, is a 593 amino acid protein. It exists several isoforms and the range of the molecular weight of isoforms are about 55-60 kDa and 29 kDa. MTF2 contains 2 PHD-type zinc fingers and belongs to the Polycomblike family. MTF2 acts by binding to H3K36me3, a mark for transcriptional activation, and recruiting the PRC2 complex, leading to enhance PRC2 H3K27me3 methylation activity and regulates the transcriptional networks during embryonic stem cell self-renewal and differentiation.

Publikationen

Species	Application	Title
mouse	WB	Nature; Polycomb-like proteins link the PRC2 complex to CpG islands.; Authors - Haojie Li ; KO Validated
mouse	WB	Nat Genet; MTF2 recruits Polycomb Repressive Complex 2 by helical-shape-selective DNA binding.; Authors - Matteo Perino
human	WB	Nat Genet; RNA is essential for PRC2 chromatin occupancy and function in human pluripotent stem cells.; Authors - Yicheng Long
human	WB	Nat Cell Biol; Targeting Menin disrupts the KMT2A/B and polycomb balance to paradoxically activate bivalent genes; Authors - Christina E Sparbier
mouse	WB	Nat Struct Mol Biol; G-tract RNA removes Polycomb repressive complex 2 from genes.; Authors - Manuel Beltran
human	WB,ChIP	Mol Cell; Competition between PRC2.1 and 2.2 subcomplexes regulates PRC2 chromatin occupancy in human stem cells.; Authors - Daniel T Youmans