MTA3 Polyklonaler Antikörper

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• KD/KO Validated

MTA3 Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

12

Kat-Nr. : 14682-1-AP

Synonyme

KIAA1266, MTA3

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Galerie der Validierungsdaten

• 

  WB analysis of HepG2 using 14682-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 14682-1-AP (MTA3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 14682-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 14682-1-AP (MTA3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 14682-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 14682-1-AP (MTA3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 14682-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 14682-1-AP (MTA3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse thymus using 14682-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 14682-1-AP (MTA3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 14682-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 14682-1-AP (MTA3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 14682-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 14682-1-AP (MTA3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse thymus using 14682-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 14682-1-AP (MTA3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 14682-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 14682-1-AP (MTA3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 14682-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 14682-1-AP (MTA3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 14682-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 14682-1-AP (MTA3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 14682-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 14682-1-AP (MTA3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 14682-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 14682-1-AP (MTA3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 14682-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14682-1-AP (MTA3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IP experiment of Raji using 14682-1-AP

  IP result of anti-MTA3(IP:14682-1-AP, 4ug; Detection:14682-1-AP 1:3000) with Raji cells lysate 2840 ug.

• 

  IHC staining of human brain using 14682-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 14682-1-AP (MTA3 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human brain using 14682-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 14682-1-AP (MTA3 antibody) at dilution of 1:100 (under 10x lens).

• 

  IF Staining of HepG2 using 14682-1-AP

  Immunofluorescent analysis of HepG2 cells, using MTA3 antibody 14682-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, Jurkat-Zellen, Maushirngewebe, Maus-Thymusgewebe, MCF-7-Zellen, Raji-Zellen
• Erfolgreiche IP: Raji-Zellen
• Erfolgreiche Detektion in IHC: humanes Hirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 6 publications below
• IHC: See 8 publications below

Produktinformation

14682-1-AP bindet in WB, IP, IHC, IF, ELISA MTA3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MTA3 fusion protein Ag6400
• Vollständiger Name: metastasis associated 1 family, member 3
• Berechnetes Molekulargewicht: 68 kDa
• Beobachtetes Molekulargewicht: 62 kDa, 68 kDa
• GenBank-Zugangsnummer: BC053631
• Gene symbol: MTA3
• Gene ID (NCBI): 57504
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Metastasis-associated protein 3 (MTA3) is a cell-type specific component of the Mi-2-NURD transcriptional co-repressor complex. It has a role in maintenance of the normal epithelial architecture through the repression of SNAI1 transcription in a histone deacetylase-dependent manner, and thus the regulation of E-cadherin levels.

Publikationen

Species	Application	Title
mouse	WB	Cell Stem Cell; The polycomb group protein L3mbtl2 assembles an atypical PRC1-family complex that is essential in pluripotent stem cells and early development.; Authors - Jinzhong Qin
mouse		Mol Cell; The Nucleosome Remodeling and Deacetylation Complex Modulates Chromatin Structure at Sites of Active Transcription to Fine-Tune Gene Expression.; Authors - Susanne Bornelöv
human	IHC	Oncotarget; Metastasis-associated protein 3 in colorectal cancer determines tumor recurrence and prognosis.; Authors - Yi Huang
human	WB,IHC	PLoS One; Overexpression of MTA3 Correlates with Tumor Progression in Non-Small Cell Lung Cancer.; Authors - Li Haiying H ; KD Validated
human	IHC	J Gastroenterol Hepatol; Overexpression of the metastasis-associated gene MTA3 correlates with tumor progression and poor prognosis in hepatocellular carcinoma.; Authors - Chuanxi Wang
human	WB,IHC	Tumour Biol; MTA3 regulates malignant progression of colorectal cancer through Wnt signaling pathway.; Authors - Taiwei Jiao