MTA2 Polyklonaler Antikörper

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• KD/KO Validated

MTA2 Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, CoIP, ChIP, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Kat-Nr. : 17554-1-AP

Synonyme

Metastasis associated 1 like 1, MTA1 L1 protein, MTA1L1, MTA2, PID

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Galerie der Validierungsdaten

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  WB analysis of HEK-293 using 17554-1-AP

  WB result of MTA2 antibody (17554-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MTA2 transfected HEK-293 cells.

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  WB analysis using 17554-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17554-1-AP (MTA2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse thymus using 17554-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 17554-1-AP (MTA2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 17554-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 17554-1-AP (MTA2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 17554-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17554-1-AP (MTA2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse thymus using 17554-1-AP

  IP Result of anti-MTA2 (IP:17554-1-AP, 4ug; Detection:17554-1-AP 1:500) with mouse thymus tissue lysate 2800ug.

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  IHC staining of human colon using 17554-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 17554-1-AP (MTA2 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human colon using 17554-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 17554-1-AP (MTA2 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human heart using 17554-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 17554-1-AP (MTA2 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human heart using 17554-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 17554-1-AP (MTA2 antibody) at dilution of 1:100 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HEK-293-Zellen, Jurkat-Zellen, Maus-Thymusgewebe
• Erfolgreiche IP: Maus-Thymusgewebe
• Erfolgreiche Detektion in IHC: humanes Kolongewebe, humanes Herzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 3 publications below
• CoIP: See 1 publications below
• ChIP: See 1 publications below

Produktinformation

17554-1-AP bindet in WB, IP, IHC, CoIP, ChIP, ELISA MTA2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MTA2 fusion protein Ag11713
• Vollständiger Name: metastasis associated 1 family, member 2
• Berechnetes Molekulargewicht: 668 aa, 75 kDa
• Beobachtetes Molekulargewicht: 70-75 kDa
• GenBank-Zugangsnummer: BC053650
• Gene symbol: MTA2
• Gene ID (NCBI): 9219
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Metastasis-associated protein MTA2, also named as MTA1L1 or PID, is a 668 amino acid protein, which contains one BAH domain, one ELM2 domain, one GATA-type zinc finger and one SANT domain. MTA2 localizes in the nucleus and is widely expressed in many tissues. MTA2 may be involved in the regulation of gene expression as repressor and activator.

Publikationen

Species	Application	Title
human	WB	Nat Commun; Identification of proximal SUMO-dependent interactors using SUMO-ID.; Authors - Orhi Barroso-Gomila
human	WB	EMBO Rep; miR-AB, a miRNA-based shRNA viral toolkit for multicolor-barcoded multiplex RNAi at a single-cell level.; Authors - Dapeng Wang
human	WB	Cell Mol Gastroenterol Hepatol; Targeting HNRNPM Inhibits Cancer Stemness and Enhances Antitumor Immunity in Wnt-activated Hepatocellular Carcinoma.; Authors - Gui-Qi Zhu ; KD Validated
	CoIP,ChIP	Cancer Sci; MTA2 promotes the metastasis of esophageal squamous cell carcinoma via EIF4E-Twist feedback loop.; Authors - Su-Li Dai