MSRB2 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

MSRB2 Polyklonal Antikörper für IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

8

Kat-Nr. : 17629-1-AP

Synonyme

CBS 1, CBS1, CGI 131, MSRB, MSRB2, PILB

---

Galerie der Validierungsdaten

• 

  WB analysis using 17629-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17629-1-AP (MSRB2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 17629-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 17629-1-AP (MSRB2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human kidney using 17629-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 17629-1-AP (MSRB2 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human kidney using 17629-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 17629-1-AP (MSRB2 Antibody) at dilution of 1:200 (under 40x lens).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, humanes Herzgewebe, Mausherzgewebe, Rattenhirngewebe
• Erfolgreiche Detektion in IHC: humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:100-1:400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 8 publications below

Produktinformation

17629-1-AP bindet in WB, IHC, ELISA MSRB2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MSRB2 fusion protein Ag11881
• Vollständiger Name: methionine sulfoxide reductase B2
• Berechnetes Molekulargewicht: 182 aa, 20 kDa
• Beobachtetes Molekulargewicht: 19 kDa
• GenBank-Zugangsnummer: BC018030
• Gene symbol: MSRB2
• Gene ID (NCBI): 22921
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Methionine sulfoxide reductase B2(MSRB2), is a 182-amino acid protein with an N-terminal mitochondrial targeting signal, a catalytic cysteine, and 2 zinc-coordinating CxxC motifs. MSRB can catalyze the stereospecific reduction of methionine-R-sulfoxides to methionines. MSRB2 protects mitochondrial integrity and cell survival by scavenging reactive oxygen species.

Publikationen

Species	Application	Title
mouse	WB	Free Radic Biol Med; Loss of methionine sulfoxide reductases increases resistance to oxidative stress.; Authors - Lo Lai
human	WB	Front Pharmacol; 3-Mercaptopyruvate Sulfurtransferase, Not Cystathionine β-Synthase Nor Cystathionine γ-Lyase, Mediates Hypoxia-Induced Migration of Vascular Endothelial Cells.; Authors - Beibei Tao ; KD Validated
rat	WB	J Med Food; Rice Protein Exerts Endogenous Antioxidant Capacity via Methionine Sulfoxide Reductase and the Nrf2 Antioxidant System Independent of Age.; Authors - Hui Li
rat	WB	Food Sci Biotechnol; Methionine strengthens anti-inflammation of rice protein via depressing NF-κB activation and stimulating Msr expression in rats fed cholesterol-enriched diets.; Authors - Zhengxuan Wang
rat	WB	J Food Biochem; Rice protein stimulates endogenous antioxidant response attributed to methionine availability in growing rats.; Authors - Zhengxuan Wang
rat	WB	Mol Omics; Rbm20 ablation is associated with changes in the expression of titin-interacting and metabolic proteins.; Authors - Eli J Larson