Moesin Polyklonaler Antikörper

Moesin Polyklonal Antikörper für FC, IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Rezensionen (1)

Kat-Nr. : 26053-1-AP

Synonyme

moesin, MSN

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Galerie der Validierungsdaten

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  WB analysis of Jurkat using 26053-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 26053-1-AP (MSN Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 26053-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26053-1-AP (MSN antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 26053-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 26053-1-AP (MSN Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse kidney using 26053-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 26053-1-AP (MSN antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 26053-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 26053-1-AP (MSN antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 26053-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 26053-1-AP (MSN antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HepG2 using 26053-1-AP

  1X10^6 HepG2 cells were intracellularly stained with 0.20ug Anti-Human MSN (26053-1-AP) (red) or 0.20 ug control antibody (blue) and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000. Fixed with 90% MeOH.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, C6-Zellen, Jurkat-Zellen, Mausherzgewebe, Mauslungengewebe, Neuro-2a-Zellen, Rattenherzgewebe
• Erfolgreiche Detektion in IHC: Mausnierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen
• Erfolgreiche Detektion in FC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:20-1:200
• Durchflusszytometrie (FC): FC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

26053-1-AP bindet in WB, IHC, IF, FC, ELISA Moesin und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Moesin fusion protein Ag23531
• Vollständiger Name: moesin
• Berechnetes Molekulargewicht: 577 aa, 68 kDa
• Beobachtetes Molekulargewicht: 68-70 kDa
• GenBank-Zugangsnummer: BC017293
• Gene symbol: MSN
• Gene ID (NCBI): 4478
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Moesin belongs to the ezrin-radixin-moesin (ERM) family of proteins which act as cross-linkers between membrane and actin cytoskeleton. ERM proteins provide structural links to strengthen the cell cortex and facilitate several key cellular processes, including membrane dynamics, substrate adhesion, cell survival, cell adhesion, and motility. The function of ERM proteins is highly reliant on phosphorylation induced conformational changes in response to growth factor, chemokine, and antigen stimulation. This antibody may cross-react with ezrin or radixin with molecular weights around 68-70 kDa.

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Caitlin (Verified Customer) (09-10-2019)

Good Antibody for western blot

• Applications: Western Blot,
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: Thyroid Cancer Cell Lines