Moesin Polyklonaler Antikörper

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• KD/KO Validated

Moesin Polyklonal Antikörper für FC, IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Kat-Nr. : 16495-1-AP

Synonyme

moesin, MSN

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Galerie der Validierungsdaten

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  WB analysis using 16495-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16495-1-AP (Moesin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 16495-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 16495-1-AP (Moesin antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 16495-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16495-1-AP (Moesin antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human placenta using 16495-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 16495-1-AP (Moesin antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human placenta using 16495-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 16495-1-AP (Moesin antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of mouse kidney using 16495-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 16495-1-AP (Moesin antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 16495-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 16495-1-AP (Moesin antibody) at dilution of 1:200 (under 40x lens) Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0).

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  IHC staining of human skin using 16495-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 16495-1-AP (Moesin antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human skin using 16495-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 16495-1-AP (Moesin antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human ovary using 16495-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 16495-1-AP (Moesin antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HepG2 using 16495-1-AP

  Immunofluorescent analysis of HepG2 cells, using MSN antibody 16495-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

• 

  IF Staining of HepG2 using 16495-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using Moesin antibody (16495-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HepG2 using 16495-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Moesin antibody (16495-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HepG2 using 16495-1-AP

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human Moesin (16495-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: BxPC-3-Zellen, C6-Zellen, HeLa-Zellen, Jurkat-Zellen, NIH/3T3-Zellen, Raji-Zellen, SGC-7901-Zellen
• Erfolgreiche Detektion in IHC: Mausnierengewebe, humanes Eierstockgewebe, humanes Hautgewebe, humanes Plazenta-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen
• Erfolgreiche Detektion in FC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:150-1:600
• Immunfluoreszenz (IF): IF : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

16495-1-AP bindet in WB, IHC, IF, FC, ELISA Moesin und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Moesin fusion protein Ag9623
• Vollständiger Name: moesin
• Berechnetes Molekulargewicht: 577 aa, 68 kDa
• Beobachtetes Molekulargewicht: 68-70 kDa
• GenBank-Zugangsnummer: BC017293
• Gene symbol: MSN
• Gene ID (NCBI): 4478
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Moesin belongs to the ezrin-radixin-moesin (ERM) family of proteins which act as cross-linkers between membrane and actin cytoskeleton. ERM proteins provide structural links to strengthen the cell cortex and facilitate several key cellular processes, including membrane dynamics, substrate adhesion, cell survival, cell adhesion, and motility. The function of ERM proteins is highly reliant on phosphorylation induced conformational changes in response to growth factor, chemokine, and antigen stimulation. This antibody may cross-react with ezrin or radixin with molecular weights around 68-70 kDa.

Publikationen

Species	Application	Title
human	IHC	Acta Neuropathol Commun; The amyloid plaque proteome in early onset Alzheimer's disease and Down syndrome.; Authors - Eleanor Drummond
human	WB	Int J Mol Sci; Bidirectional Tumor-Promoting Activities of Macrophage Ezrin.; Authors - Krishnendu Khan ; KD Validated
human	WB	Front Cell Dev Biol; Ectopic Endometrial Cell-Derived Exosomal Moesin Induces Eutopic Endometrial Cell Migration, Enhances Angiogenesis and Cytosolic Inflammation in Lesions Contributes to Endometriosis Progression.; Authors - Maidinaimu Abudula
mouse	IF	Am J Pathol; Elevated Expression of Moesin in Muscular Dystrophies.; Authors - Mark Pines