MSMP Polyklonaler Antikörper

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• KD/KO Validated

MSMP Polyklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 19526-1-AP

Synonyme

MSMP, PC 3, PC3 secreted microprotein, PSMP

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  WB analysis of DU 145 using 19526-1-AP

  WB result of MSMP antibody (19526-1-AP; 1:400; incubated at room temperature for 1.5 hours) with sh-Control and sh-MSMP transfected DU 145 cells.

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  WB analysis using 19526-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 19526-1-AP (MSMP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis using 19526-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19526-1-AP (MSMP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IF Staining of PC-3 using 19526-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed PC-3 cells using MSMP antibody (19526-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: PC-3-Zellen, DU 145-Zellen
• Erfolgreiche Detektion in IF/ICC: PC-3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

19526-1-AP bindet in WB, IF/ICC, ELISA MSMP und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: microseminoprotein, prostate associated
• Berechnetes Molekulargewicht: 15 kDa
• Beobachtetes Molekulargewicht: 12 kDa, 20 kDa
• GenBank-Zugangsnummer: NM_001044264
• Gene symbol: MSMP
• Gene ID (NCBI): 692094
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MSMP, also named PSMP, belongs to the beta-microseminoprotein family. MSMP is highly conserved, sharing 91 to 94% amino acid identity with its rodent and dog homologs. RT-PCR of 27 human tissues detected PSMP expression only in trachea and, to a lesser extent, testis. MSMP was highly expressed in PC3 cells, but not in 2 other prostate cancer cell lines. Western blot analysis revealed 20 kDa and 12 kDa bands PSMP maybe due to posttranslational modification.