MSH6 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

MSH6 Polyklonal Antikörper für FC, IF, IHC, IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

7

Kat-Nr. : 18120-1-AP

Synonyme

G/T mismatch binding protein, GTBP, GTMBP, hMSH6, HNPCC5, HSAP, MSH6, MutS alpha 160 kDa subunit, mutS homolog 6 (E. coli), p160

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Galerie der Validierungsdaten

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  WB analysis of HEK-293 using 18120-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 18120-1-AP (MSH6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 18120-1-AP

  WB result of MSH6 antibody (18120-1-AP, 1:6000) with si-Control and si-MSH6 transfected HEK293 cells.

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  WB analysis of HEK-293 using 18120-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 18120-1-AP (MSH6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 18120-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 18120-1-AP (MSH6 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of COLO 320 using 18120-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 18120-1-AP (MSH6 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 18120-1-AP

  IP Result of anti-MSH6 (IP:18120-1-AP, 4ug; Detection:18120-1-AP 1:600) with HEK-293 cells lysate 1120ug.

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  IHC staining of human colon using 18120-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 18120-1-AP (MSH6 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human colon using 18120-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 18120-1-AP (MSH6 Antibody) at dilution of 1:200 (under 10x lens).

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  IF Staining of HeLa using 18120-1-AP

  Immunofluorescent analysis of Hela cells, using MSH6 antibody 18120-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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  FC experiment of HEK-293 using 18120-1-AP

  1X10^6 HEK-293 cells were intracellularly stained with 0.2 ug Anti-Human MSH6 (18120-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (green), and 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with 0.1% TritonX-100.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HEK-293
• Erfolgreiche IP: HEK-293-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolongewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen
• Erfolgreiche Detektion in FC: HEK-293-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:10-1:100
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 5 publications below
• IHC: See 2 publications below
• IP: See 1 publications below

Produktinformation

18120-1-AP bindet in WB, IP, IHC, IF, FC, ELISA MSH6 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MSH6 fusion protein Ag12645
• Vollständiger Name: mutS homolog 6 (E. coli)
• Berechnetes Molekulargewicht: 153 kDa
• Beobachtetes Molekulargewicht: 150-160 kDa
• GenBank-Zugangsnummer: BC004246
• Gene symbol: MSH6
• Gene ID (NCBI): 2956
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MSH6, also named as DNA mismatch repair protein Msh6 or GTMBP, is a 1360 amino acid protein, which contains one PWWP domain and belongs to the DNA mismatch repair MutS family. MSH6 localizes in the nucleus and is a component of the post-replicative DNA mismatch repair system. Msh2 and Msh6 form a protein complex required to repair mismatches generated during DNA replication.

Publikationen

Species	Application	Title
human	WB	Mol Cancer; CircLIFR synergizes with MSH2 to attenuate chemoresistance via MutSα/ATM-p73 axis in bladder cancer.; Authors - Hui Zhang
human	WB	Oncotarget; Targeted silencing of SOX2 by an artificial transcription factor showed antitumor effect in lung and esophageal squamous cell carcinoma.; Authors - Etsuko Yokota
human	IHC	Front Oncol; Molecular Classification in Patients With Endometrial Cancer After Fertility-Preserving Treatment: Application of ProMisE Classifier and Combination of Prognostic Evidence.; Authors - Xuting Ran
human	WB	DNA Cell Biol; MSH6 Aggravates the Hypoxic Microenvironment via Regulating HIF1A to Promote the Metastasis of Glioblastoma Multiforme.; Authors - Ying Shi
mouse	WB	Nucleic Acids Res; Resolution of sequence divergence for repeat-mediated deletions shows a polarity that is mediated by MLH1; Authors - Hannah Trost
human	IHC	Front Oncol; The MSH2 c.793-1G>A variant disrupts normal splicing and is associated with Lynch syndrome; Authors - Yiming Li