MRPS35 Polyklonaler Antikörper

MRPS35 Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

28

Kat-Nr. : 16457-1-AP

Synonyme

HDCMD11P, MDS023, MRP S28, MRP S35, MRPS28, MRPS35, PSEC0213, S28mt, S35mt

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Galerie der Validierungsdaten

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  WB analysis using 16457-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16457-1-AP (MRPS35 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 16457-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16457-1-AP (MRPS35 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 16457-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 16457-1-AP (MRPS35 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 16457-1-AP

  IP Result of anti-MRPS35 (IP:16457-1-AP, 4ug; Detection:16457-1-AP 1:1000) with HeLa cells lysate 1200ug.

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  IHC staining of human colon cancer using 16457-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16457-1-AP (MRPS35 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 16457-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16457-1-AP (MRPS35 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 16457-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using MRPS35 antibody (16457-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HepG2-Zellen, humanes Herzgewebe
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 28 publications below
• IP: See 1 publications below

Produktinformation

16457-1-AP bindet in WB, IP, IHC, IF, ELISA MRPS35 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MRPS35 fusion protein Ag9430
• Vollständiger Name: mitochondrial ribosomal protein S35
• Berechnetes Molekulargewicht: 323 aa, 37 kDa
• Beobachtetes Molekulargewicht: 37 kDa
• GenBank-Zugangsnummer: BC015862
• Gene symbol: MRPS35
• Gene ID (NCBI): 60488
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Mammalian mitochondrial ribosomes (mitoribosomes) are responsible for protein synthesis within the mitochondrion. The mitoribosomes are composed of a 4:1 ratio of protein to RNA, with the proteins forming two subunits, the 28S subunit and the 39S subunit. Across species, the proteins that make up the mitoribosome subunits vary greatly in sequence, preventing easy recognition by sequence homology. MRPS35 (mitochondrial ribosomal protein S35), also known as MDS023, MRPS28 or HDCMD11P, is a 323 amino acid protein that localizes to the mitochondrion, where it exists as a component of the 28S ribosomal subunit and works in conjunction with other MRPs to mediate protein synthesis. Existing as two alternatively spliced isoforms, MRPS35 is encoded by a gene located on human chromosome 10, which houses over 1,200 genes and comprises nearly 4.5% of the human genome. This antibody is a rabbit polyclonal antibody raised against the full length MRPS35 protein. It specifically reacts with the 37kd MRPS35protein.

Publikationen

Species	Application	Title
mouse	WB	Cell Metab; Integrative genetic analysis identifies FLVCR1 as a plasma-membrane choline transporter in mammals; Authors - Timothy C Kenny
mouse	WB	Sci Adv; Adult-onset obesity is triggered by impaired mitochondrial gene expression.; Authors - Kara L Perks
mouse	WB	Sci Adv; Fidelity of translation initiation is required for coordinated respiratory complex assembly.; Authors - Danielle L Rudler
	WB	Cell Metab; Tissue-Specific Loss of DARS2 Activates Stress Responses Independently of Respiratory Chain Deficiency in the Heart.; Authors - Sukru Anil Dogan
mouse	WB	J Clin Invest; AFG3L2 supports mitochondrial protein synthesis and Purkinje cell survival.; Authors - Almajan Eva R ER
mouse	WB	Nat Commun; Loss of the RNA-binding protein TACO1 causes late-onset mitochondrial dysfunction in mice.; Authors - Tara R Richman