MRPS15 Polyklonaler Antikörper

MRPS15 Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

12

Kat-Nr. : 17006-1-AP

Synonyme

DC37, MPR S15, MRP S15, MRPS15, RPMS15, S15mt

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Galerie der Validierungsdaten

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  WB analysis using 17006-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17006-1-AP (MRPS15 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 17006-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 17006-1-AP (MRPS15 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 17006-1-AP

  IP result of anti-MRPS15 (IP:17006-1-AP, 4ug; Detection:17006-1-AP 1:300) with mouse brain tissue lysate 3200 ug.

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  IHC staining of human spleen using 17006-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 17006-1-AP (MRPS15 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human spleen using 17006-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 17006-1-AP (MRPS15 antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of HepG2 using 17006-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using MRPS15 rabbit poly (17006-1-AP, Green) and KRT18 mouse mAb (66187-1-Ig, Red) at dilution of 1:50, further stained with Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) for 17006-1-AP, and Alexa Fluor 594-conjugated AffiniPure Goat Anti-Mouse IgG (H+L) for 66187-1-Ig.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, Mauslebergewebe, MCF-7-Zellen, Raji-Zellen
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Milzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 11 publications below

Produktinformation

17006-1-AP bindet in WB, IP, IHC, IF, ELISA MRPS15 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MRPS15 fusion protein Ag10702
• Vollständiger Name: mitochondrial ribosomal protein S15
• Berechnetes Molekulargewicht: 257 aa, 30 kDa
• Beobachtetes Molekulargewicht: 25-30 kDa
• GenBank-Zugangsnummer: BC031336
• Gene symbol: MRPS15
• Gene ID (NCBI): 64960
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MRPS15, also named as RPMS15 or DC37, is a 257 amino acid protein, which belongs to the ribosomal protein S15P family. MRPS15 localizes in the Mitochondrion and is a component of the mitochondrial ribosome small subunit (28S) which comprises a 12S rRNA and about 30 distinct proteins. MRPS15 is involved into the mitochondrial proteins biosynthesis and ribosomal biogenesis.

Publikationen

Species	Application	Title
human	WB	Cell; An in vitro system to silence mitochondrial gene expression.; Authors - Luis Daniel Cruz-Zaragoza
human	WB	Cell; Mitochondrial Protein Synthesis Adapts to Influx of Nuclear-Encoded Protein.; Authors - Ricarda Richter-Dennerlein
human	WB	Cell Metab; Initial steps in RNA processing and ribosome assembly occur at mitochondrial DNA nucleoids.; Authors - Daniel F Bogenhagen
human	WB	Nucleic Acids Res; The human Obg protein GTPBP10 is involved in mitoribosomal biogenesis.; Authors - Elena Lavdovskaia
human	WB	Hum Mol Genet; Mutations in the MRPS28 gene encoding the small mitoribosomal subunit protein bS1m in a patient with intrauterine growth retardation, craniofacial dysmorphism and multisystemic involvement.; Authors - Juliette Pulman
human	WB	J Biol Chem; Mitochondrial rRNA Methyltransferase Family Members are Positioned to Modify Nascent rRNA in Foci Near the mtDNA Nucleoid.; Authors - Lee Ken-Wing KW