MRPL38 Polyklonaler Antikörper

MRPL38 Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 15913-1-AP

Synonyme

HSPC262, KIAA1863, L38mt, MRP L3, MRP L38, MRPL38, RPML3

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Galerie der Validierungsdaten

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  WB analysis using 15913-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 15913-1-AP (MRPL38 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 15913-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 15913-1-AP (MRPL38 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 15913-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 15913-1-AP (MRPL38 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 15913-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 15913-1-AP (MRPL38 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 15913-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 15913-1-AP (MRPL38 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 15913-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 15913-1-AP (MRPL38 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 15913-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15913-1-AP (MRPL38 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 15913-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15913-1-AP (MRPL38 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 15913-1-AP

  Immunofluorescent analysis of HepG2 cells, using MRPL38 antibody 15913-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, humanes Plazenta-Gewebe, Mauslebergewebe
• Erfolgreiche Detektion in IHC: humanes Lebergewebe, Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IHC: See 1 publications below

Produktinformation

15913-1-AP bindet in WB, IHC, IF, ELISA MRPL38 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MRPL38 fusion protein Ag8706
• Vollständiger Name: mitochondrial ribosomal protein L38
• Berechnetes Molekulargewicht: 346 aa, 41 kDa
• Beobachtetes Molekulargewicht: 37-45 kDa
• GenBank-Zugangsnummer: BC013311
• Gene symbol: MRPL38
• Gene ID (NCBI): 64978
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
mouse	WB	Int J Mol Sci; Translation Fidelity and Respiration Deficits in CLPP-Deficient Tissues: Mechanistic Insights from Mitochondrial Complexome Profiling; Authors - Jana Key
rat	WB	Cell Rep; Small RNAs derived from tRNA fragmentation regulate the functional maturation of neonatal β cells.; Authors - Mustafa Bilal Bayazit
	IHC	J Toxicol Sci; Aberrant epigenetic gene regulation in hippocampal neurogenesis of mouse offspring following maternal exposure to 3,3'-iminodipropionitrile.; Authors - Takeshi Tanaka