MRPL13 Polyklonaler Antikörper

MRPL13 Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Affe, human, Maus, Ratte

Anwendung

WB, IP, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

7

Kat-Nr. : 16241-1-AP

Synonyme

L13, L13A, L13mt, MRP L13, MRPL13, RPL13, RPML13

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Galerie der Validierungsdaten

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  WB analysis using 16241-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16241-1-AP (MRPL13 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat lung using 16241-1-AP

  rat lung tissue were subjected to SDS PAGE followed by western blot with 16241-1-AP (MRPL13 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 16241-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16241-1-AP (MRPL13 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HL-60 using 16241-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 16241-1-AP (MRPL13 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 16241-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 16241-1-AP (MRPL13 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 16241-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 16241-1-AP (MRPL13 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COS-7 using 16241-1-AP

  COS-7 cells were subjected to SDS PAGE followed by western blot with 16241-1-AP (MRPL13 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse lung using 16241-1-AP

  IP Result of anti-MRPL13 (IP:16241-1-AP, 4ug; Detection:16241-1-AP 1:500) with mouse lung tissue lysate 4000ug.

• 

  IHC staining of human liver cancer using 16241-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16241-1-AP (MRPL13 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 16241-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16241-1-AP (MRPL13 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 16241-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16241-1-AP (MRPL13 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 16241-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16241-1-AP (MRPL13 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HL-60-Zellen, COS-7-Zellen, HeLa-Zellen, Jurkat-Zellen, Mauslungengewebe, Rattenlungengewebe
• Erfolgreiche IP: Mauslungengewebe
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe, humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 6 publications below
• IHC: See 1 publications below

Produktinformation

16241-1-AP bindet in WB, IP, IHC, ELISA MRPL13 und zeigt Reaktivität mit Affe, human, Maus, Ratten

• Getestete Reaktivität: Affe, human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MRPL13 fusion protein Ag9105
• Vollständiger Name: mitochondrial ribosomal protein L13
• Berechnetes Molekulargewicht: 178 aa, 21 kDa
• Beobachtetes Molekulargewicht: 20-23 kDa
• GenBank-Zugangsnummer: BC009190
• Gene symbol: MRPL13
• Gene ID (NCBI): 28998
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MRPL13, also named as 39S ribosomal protein L13, mitochondrial or L13mt, is a 178 amino acid protein, which belongs to the ribosomal protein L13P family. MRPL13 localizes in the mitochondrion and interacts with OXA1L. MRPL13 is involved in mitochondrial proteins biosynthesis, ribosomal structure and biogenesis.

Publikationen

Species	Application	Title
human	WB	Nucleic Acids Res; The human Obg protein GTPBP10 is involved in mitoribosomal biogenesis.; Authors - Elena Lavdovskaia
human	WB	Nucleic Acids Res; Transcription-independent role for human mitochondrial RNA polymerase in mitochondrial ribosome biogenesis.; Authors - Surovtseva Yulia V YV
human	WB	Nucleic Acids Res; MPV17L2 is required for ribosome assembly in mitochondria.; Authors - Ilaria Dalla Rosa
human	WB	Genomics Proteomics Bioinformatics; Integrative Multi-omics Landscape of Non-structural Protein 3 of Severe Acute Respiratory Syndrome Coronaviruses.; Authors - Ruona Shi
human	WB	J Cell Sci; CLUH couples mitochondrial distribution to the energetic and metabolic status.; Authors - Jamal Wakim
human	WB	Life (Basel); Molecular Investigation of Mitochondrial RNA19 Role in the Pathogenesis of MELAS Disease; Authors - Paola Loguercio Polosa