MRPL11 Polyklonaler Antikörper

MRPL11 Polyklonal Antikörper für FC, IF, IHC, IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, FC, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

12

Kat-Nr. : 15543-1-AP

Synonyme

CGI 113, L11MT, MRP L11, MRPL11

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Galerie der Validierungsdaten

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  WB analysis using 15543-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15543-1-AP (MRPL11 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse liver using 15543-1-AP

  IP Result of anti-MRPL11 (IP:15543-1-AP, 4ug; Detection:15543-1-AP 1:1000) with mouse liver tissue lysate 4000ug.

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  IHC staining of human liver using 15543-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 15543-1-AP (MRPL11 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human liver using 15543-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 15543-1-AP (MRPL11 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HepG2 using 15543-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 15543-1-AP (MRPL11 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of HepG2 using 15543-1-AP

  1X10^6 HepG2 cells were stained with 0.2ug MRPL11 antibody (15543-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: BxPC-3-Zellen, K-562-Zellen
• Erfolgreiche IP: Mauslebergewebe
• Erfolgreiche Detektion in IHC: humanes Lebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen
• Erfolgreiche Detektion in FC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 12 publications below
• CoIP: See 1 publications below

Produktinformation

15543-1-AP bindet in WB, IP, IHC, IF, FC, CoIP, ELISA MRPL11 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MRPL11 fusion protein Ag7899
• Vollständiger Name: mitochondrial ribosomal protein L11
• Berechnetes Molekulargewicht: 21 kDa
• Beobachtetes Molekulargewicht: 21 kDa
• GenBank-Zugangsnummer: BC005002
• Gene symbol: MRPL11
• Gene ID (NCBI): 65003
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MRPL11 is one of the protein components of mitochondrial ribosomes encoded by the nuclear genome, which is essential for oxidative phosphorylation.

Publikationen

Species	Application	Title
mouse	WB	Sci Adv; Adult-onset obesity is triggered by impaired mitochondrial gene expression.; Authors - Kara L Perks
human	WB	Nat Commun; RNA modification landscape of the human mitochondrial tRNALys regulates protein synthesis.; Authors - Uwe Richter
mouse	WB	J Cell Biol; Quality control of mitochondrial protein synthesis is required for membrane integrity and cell fitness.; Authors - Uwe Richter
human	WB	Am J Hum Genet; Biallelic Mutations in MRPS34 Lead to Instability of the Small Mitoribosomal Subunit and Leigh Syndrome.; Authors - Nicole J Lake
human	WB	Hum Mol Genet; Translation of MT-ATP6 pathogenic variants reveals distinct regulatory consequences from the co-translational quality control of mitochondrial protein synthesis.; Authors - Kah Ying Ng
human	WB	Hum Mol Genet; A variant in MRPS14 (uS14m) causes perinatal hypertrophic cardiomyopathy with neonatal lactic acidosis, growth retardation, dysmorphic features and neurological involvement.; Authors - Christopher B Jackson