MPP5 Polyklonaler Antikörper

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• KD/KO Validated

MPP5 Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

22

Kat-Nr. : 17710-1-AP

Synonyme

MAGUK p55 subfamily member 5, MPP5, PALS1

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Galerie der Validierungsdaten

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  WB analysis using 17710-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17710-1-AP (MPP5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis using 17710-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17710-1-AP (MPP5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 17710-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 17710-1-AP (MPP5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 17710-1-AP

  IP Result of anti-MPP5 (IP:17710-1-AP, 3ug; Detection:17710-1-AP 1:1000) with mouse brain tissue lysate 8000ug.

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  IHC staining of mouse brain using 17710-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17710-1-AP (MPP5 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 17710-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17710-1-AP (MPP5 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: DU 145-Zellen, HeLa-Zellen, humanes Hirngewebe, Y79-Zellen
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 5 publications below
• IHC: See 6 publications below
• IF: See 15 publications below
• IP: See 1 publications below

Produktinformation

17710-1-AP bindet in WB, IP, IHC, IF, ELISA MPP5 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MPP5 fusion protein Ag12010
• Vollständiger Name: membrane protein, palmitoylated 5 (MAGUK p55 subfamily member 5)
• Berechnetes Molekulargewicht: 675 aa, 77 kDa
• Beobachtetes Molekulargewicht: 70-80 kDa
• GenBank-Zugangsnummer: BC095485
• Gene symbol: MPP5
• Gene ID (NCBI): 64398
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MPP5, also named as PALS1, belongs to the MAGUK family which interact with the cytoskeleton and regulate cell proliferation, signaling pathways, and intracellular junctions. MPP5 is thought to organize intracellular PALS1-CRB-MUPP1 protein scaffolds in the retina that are involved in maintenance of photoreceptor-Mu¨ller glia cell adhesion. MPP5 plays a role in tight junctions biogenesis and in the establishment of cell polarity in epithelial cells.

Publikationen

Species	Application	Title
mouse	IF	Neuron; Microcephaly Proteins Wdr62 and Aspm Define a Mother Centriole Complex Regulating Centriole Biogenesis, Apical Complex, and Cell Fate.; Authors - Divya Jayaraman
mouse	IF	Neuron; Mosaic Analysis with Double Markers Reveals Distinct Sequential Functions of Lgl1 in Neural Stem Cells.; Authors - Robert Beattie
human	WB,IP	Nat Commun; CD13 orients the apical-basal polarity axis necessary for lumen formation.; Authors - Li-Ting Wang
mouse	IF	Dev Cell; Multicellular rosettes link mesenchymal-epithelial transition to radial intercalation in the mouse axial mesoderm; Authors - Marissa L Gredler
mouse	IF	Cell Death Differ; The evolutionary history of the polyQ tract in huntingtin sheds light on its functional pro-neural activities.; Authors - Raffaele Iennaco
human	WB,IF	J Am Soc Nephrol; Crumbs2 Is an Essential Slit Diaphragm Protein of the Renal Filtration Barrier.; Authors - Annika Möller-Kerutt