MPO Rekombinanter Antikörper

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• KD/KO Validated

MPO Rekombinant Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

CloneNo.

3J17

Kat-Nr. : 81610-1-RR

Synonyme

MPO, myeloperoxidase

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Galerie der Validierungsdaten

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  WB analysis of HL-60 using 81610-1-RR

  WB result of MPO antibody (81610-1-RR; 1:40000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MPO transfected HL-60 cells.

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  WB analysis of HL-60 using 81610-1-RR

  HL-60 cells were subjected to SDS PAGE followed by western blot with 81610-1-RR (MPO antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of human saliva using 81610-1-RR

  human saliva were subjected to SDS PAGE followed by western blot with 81610-1-RR (MPO antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 81610-1-RR

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 81610-1-RR (MPO antibody) at dilution of 1:1600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 81610-1-RR

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 81610-1-RR (MPO antibody) at dilution of 1:1600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HL-60 using 81610-1-RR

  Immunofluorescent analysis of (4% PFA) fixed HL-60 cells using MPO antibody (81610-1-RR, Clone: 3J17 ) at dilution of 1:1600 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HL-60-Zellen, human saliva
• Erfolgreiche Detektion in IHC: humanes Lebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HL-60-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:800-1:3200
• Immunfluoreszenz (IF): IF : 1:800-1:3200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

81610-1-RR bindet in WB, IHC, IF, ELISA MPO und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: MPO fusion protein Ag17564
• Vollständiger Name: myeloperoxidase
• Berechnetes Molekulargewicht: 745 aa, 84 kDa
• Beobachtetes Molekulargewicht: 59 kDa, 90 kDa
• GenBank-Zugangsnummer: BC130476
• Gene symbol: MPO
• Gene ID (NCBI): 4353
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The MPO gene encodes myeloperoxidase, a lysosomal hemoprotein located in the azurophilic granules of polymorphonuclear (PMN) leukocytes and monocytes. In response to stimulation, MPO is activated into a transient intermediate with potent antimicrobial oxidizing abilities(PMID:17650507). The mRNA is translated into a single protein of 90 kDa, which displays enzymatic activity and undergoes proteolytic maturation into a heavy chain of 59 kDa and a light chain of 13.5 kDa; these subunits then dimerize into the mature tetramer and the mature MPO is a heterotetramer composed of two identical heavy chains and two identical light chains(PMID:12773517). Fragments with molecular masses of 43-47 kDa were formed by autocatalysis during warming in sample buffer (PMID:12960244).The 24-kDa material had a map identical to that of 13.5 kDa subunit and represents a dimer of the 13.5 kDa subunit (PMID:3008892). Defects in MPO are the cause of myeloperoxidase deficiency (MPOD). It has 3 isoforms produced by alternative splicing.