MOV10 Polyklonaler Antikörper

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• KD/KO Validated

MOV10 Polyklonal Antikörper für FC (Intra), IF

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

IF, FC (Intra)

Konjugation

CoraLite®594 Fluorescent Dye

Kat-Nr. : CL594-10370

Synonyme

DKFZp667O1423, FLJ32791, fSAP113, gb110, KIAA1631, MOV10, Putative helicase MOV 10

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Galerie der Validierungsdaten

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  IF Staining of HepG2 using CL594-10370

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using CoraLite®594 MOV10 antibody (CL594-10370) at dilution of 1:200.

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  FC experiment of HeLa using CL594-10370

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human MOV10 (CL594-10370) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF: HepG2-Zellen
• Erfolgreiche Detektion in FC: HeLa-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF): IF : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL594-10370 bindet in IF, FC (Intra) MOV10 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MOV10 fusion protein Ag0384
• Vollständiger Name: Mov10, Moloney leukemia virus 10, homolog (mouse)
• Berechnetes Molekulargewicht: 114 kDa
• Beobachtetes Molekulargewicht: 110-115 kDa
• GenBank-Zugangsnummer: BC002548
• Gene symbol: MOV10
• Gene ID (NCBI): 4343
• Konjugation: CoraLite®594 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 588 nm / 604 nm
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MOV10, also named as KIAA1631, belongs to the DNA2/NAM7 helicase family and SDE3 subfamily. It is required for RNA-mediated gene silencing by the RNA-induced silencing complex (RISC). Human MOV10 may regulate a wide range of RNA viruses and could also control the retrotransposition of endogenous retroelements in mammals(PMID:22727223). MOV10 has a broad antiretroviral activity that can target a wide range of retroviruses, and it could be actively involved in host defense against retroviral infection. MOV10 can potently inhibit HIV-1 replication at multiple stages(PMID:20215113). It is involved in the progression of telomerase-catalyzing reaction via the interaction of telomerase protein and telomere DNA.