MORC3 Polyklonaler Antikörper

MORC3 Polyklonal Antikörper für IF, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IP, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Kat-Nr. : 24994-1-AP

Synonyme

KIAA0136, MORC3, NXP2, ZCW5, ZCWCC3

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Galerie der Validierungsdaten

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  WB analysis of HeLa using 24994-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 24994-1-AP (MORC3 Antibody) at dilution of 1:1000 incubated at 4 degree celsius over night.

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  IP experiment of HeLa using 24994-1-AP

  IP Result of anti-MORC3 (IP:24994-1-AP, 4ug; Detection:24994-1-AP 1:300) with HeLa cells lysate 4000ug.

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  IF Staining of A431 using 24994-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using MORC3 antibody (24994-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), (CL594-Phalloidin, red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IF: A431-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunfluoreszenz (IF): IF : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IF: See 1 publications below

Produktinformation

24994-1-AP bindet in WB, IP, IF, ELISA MORC3 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MORC3 fusion protein Ag21825
• Vollständiger Name: MORC family CW-type zinc finger 3
• Berechnetes Molekulargewicht: 939 aa, 107 kDa
• Beobachtetes Molekulargewicht: 130 kDa
• GenBank-Zugangsnummer: BC132731
• Gene symbol: MORC3
• Gene ID (NCBI): 23515
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MORC3, also named as MORC family CW-type zinc finger protein 3 or NXP2, is a 939 amino acid protein, which contains 1 CW-type zinc finger. MORC3 localizes in the nucleus and are expressed in heart, placenta, skeletal muscle, brain, pancreas, lung, liver, but not kidney. MORC3 as Nuclear factor which forms MORC3-NBs (nuclear bodies) via an ATP-dependent mechanism. Sumoylated MORC3-NBs can also associate with PML-NBs and recruits TP53 and SP100 to PML-NBs, thus regulating TP53 activity. It may be required for influenza A transcription during viral infection. The calculated molecular weight of MORC3 is a 107 kDa, but the Sumoylated MORC3 protein is about 130 kDa.

Publikationen

Species	Application	Title
human	WB	Nature; Self-guarding of MORC3 enables virulence factor-triggered immunity.; Authors - Moritz M Gaidt
human	WB,IF	J Med Virol; MORC3 restricts human cytomegalovirus infection by suppressing the major immediate-early promoter activity.; Authors - Dong Li