MOCS3 Polyklonaler Antikörper

MOCS3 Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 27501-1-AP

Synonyme

MOCS3, UBA4

---

Galerie der Validierungsdaten

• 

  WB analysis of MCF-7 using 27501-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 27501-1-AP (MOCS3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 27501-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 27501-1-AP (MOCS3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 27501-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 27501-1-AP (MOCS3 antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human liver cancer using 27501-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 27501-1-AP (MOCS3 antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human heart using 27501-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 27501-1-AP (MOCS3 antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human heart using 27501-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 27501-1-AP (MOCS3 antibody) at dilution of 1:200 (under 40x lens).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, MCF-7-Zellen
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe, humanes Herzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

27501-1-AP bindet in WB, IHC, ELISA MOCS3 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MOCS3 fusion protein Ag26596
• Vollständiger Name: molybdenum cofactor synthesis 3
• Berechnetes Molekulargewicht: 50 kDa
• Beobachtetes Molekulargewicht: 50 kDa, 65 kDa
• GenBank-Zugangsnummer: BC015939
• Gene symbol: MOCS3
• Gene ID (NCBI): 27304
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MOCS3, also named as molybdenum cofactor synthesis 3 and UBA4. The human MOCS3 protein contains an N-terminal domain similar to the Escherichia coli MoeB protein and a C-terminal segment displaying similarities to the sulfurtransferase rhodanese. MOCS3 is proposed to catalyze both the adenylation and the subsequent generation of a thiocarboxylate group at the C-terminus of the smaller subunit of molybdopterin (MPT) synthase during Moco biosynthesis in humans (PMID: 15910006). MOCS3 has a calculated molecular weight of 50 kDa and can be detected another band of 65 kDa due to urmylation (PMID: 21209336).